Massively parallel C. elegans tracking provides multi-dimensional fingerprints for phenotypic discovery.
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Authors
Challa, Pavan K
Kirkegaard, Julius B
Koopman, Mandy
Hardenberg, Maarten C
Müller, Thomas
Saar, Kadi L
Roode, Lianne WY
Habchi, Johnny
Vecchi, Giulia
Fernando, Nilumi
Casford, Samuel
Nollen, Ellen AA
Dobson, Christopher M
Knowles, Tuomas PJ
Publication Date
2018-08-01Journal Title
J Neurosci Methods
ISSN
0165-0270
Publisher
Elsevier BV
Volume
306
Pages
57-67
Language
eng
Type
Article
This Version
AM
Physical Medium
Print-Electronic
Metadata
Show full item recordCitation
Perni, M., Challa, P. K., Kirkegaard, J. B., Limbocker, R., Koopman, M., Hardenberg, M. C., Sormanni, P., et al. (2018). Massively parallel C. elegans tracking provides multi-dimensional fingerprints for phenotypic discovery.. J Neurosci Methods, 306 57-67. https://doi.org/10.1016/j.jneumeth.2018.02.005
Abstract
BACKGROUND: The nematode worm C. elegans is a model organism widely used for studies of genetics and of human disease. The health and fitness of the worms can be quantified in different ways, such as by measuring their bending frequency, speed or lifespan. Manual assays, however, are time consuming and limited in their scope providing a strong motivation for automation. NEW METHOD: We describe the development and application of an advanced machine vision system for characterising the behaviour of C. elegans, the Wide Field-of-View Nematode Tracking Platform (WF-NTP), which enables massively parallel data acquisition and automated multi-parameter behavioural profiling of thousands of worms simultaneously. RESULTS: We screened more than a million worms from several established models of neurodegenerative disorders and characterised the effects of potential therapeutic molecules for Alzheimer's and Parkinson's diseases. By using very large numbers of animals we show that the sensitivity and reproducibility of behavioural assays is very greatly increased. The results reveal the ability of this platform to detect even subtle phenotypes. COMPARISON WITH EXISTING METHODS: The WF-NTP method has substantially greater capacity compared to current automated platforms that typically either focus on characterising single worms at high resolution or tracking the properties of populations of less than 50 animals. CONCLUSIONS: The WF-NTP extends significantly the power of existing automated platforms by combining enhanced optical imaging techniques with an advanced software platform. We anticipate that this approach will further extend the scope and utility of C. elegans as a model organism.
Keywords
Animals, Caenorhabditis elegans, Neurodegenerative Diseases, Disease Models, Animal, Data Interpretation, Statistical, Reproducibility of Results, Drug Evaluation, Preclinical, Behavior, Animal, Phenotype, Software, Pattern Recognition, Automated, Optical Imaging, Machine Learning
Sponsorship
EPSRC (1510942)
Biotechnology and Biological Sciences Research Council (BB/J002119/1)
Identifiers
External DOI: https://doi.org/10.1016/j.jneumeth.2018.02.005
This record's URL: https://www.repository.cam.ac.uk/handle/1810/278494
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International
Licence URL: http://creativecommons.org/licenses/by-nc-nd/4.0/
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