Proteins Encoded by the gerP Operon Are Localized to the Inner Coat in Bacillus cereus Spores and Are Dependent on GerPA and SafA for Assembly.
Applied and environmental microbiology
American Society for Microbiology
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Ghosh, A., Manton, J., Mustafa, A., Gupta, M., Ayuso-Garcia, A., Rees, E., & Christie, G. (2018). Proteins Encoded by the gerP Operon Are Localized to the Inner Coat in Bacillus cereus Spores and Are Dependent on GerPA and SafA for Assembly.. Applied and environmental microbiology, 84 (14) https://doi.org/10.1128/aem.00760-18
Germination of Bacillus spores is triggered by certain amino acids and sugar molecules, which permeate through the outermost layers of the spore to interact with receptor complexes that reside in the inner membrane. Previous studies have shown that mutations in the hexacistronic gerP locus reduce the rate of spore germination, with experimental evidence indicating that the defect stems from reduced permeability of the spore coat to germinant molecules. Here we use the ellipsoid localisation microscopy technique to reveal that all six Bacillus cereus GerP proteins share proximity with cortex lytic enzymes within the inner coat. We reveal also that the GerPA protein alone can localise in the absence of all other GerP proteins, and that it has an essential role for the localisation of all other GerP proteins within the spore. The latter is also demonstrated to be SafA - but not CotE - dependent for localisation, which is consistent with an inner coat location. GerP null spores are shown also to have reduced permeability to fluorescently labelled dextran molecules compared to wild type spores. Overall, the results support the hypothesis that the GerP proteins have a structural role within the spore associated with coat permeability.
Cell Wall, Bacillus cereus, Spores, Bacterial, Bacterial Proteins, Gene Expression Regulation, Bacterial, Mutation, Genes, Bacterial, Operon, Permeability
1. Engineering and Physical Sciences Research Council Centre for Doctoral Training in Sensor Technologies and Applications (EP/L015889/1) 2. Cambridge Nehru Scholarship 3. Raymond and Beverly Sackler Foundation 4. MedImmune
External DOI: https://doi.org/10.1128/aem.00760-18
This record's URL: https://www.repository.cam.ac.uk/handle/1810/278597