Research data supporting “Towards Cellular Sieving: Exploring the Limits of Scaffold Accessibility for Cell Type Specific Invasion” (Version 2)
Authors
Ashworth, Jennifer C
Mehr, Marco
Buxton, Paul G
Best, Serena M
Cameron, Ruth E
Publication Date
2018-08-14Type
Dataset
Metadata
Show full item recordCitation
Ashworth, J. C., Mehr, M., Buxton, P. G., Best, S. M., & Cameron, R. E. (2018). Research data supporting “Towards Cellular Sieving: Exploring the Limits of Scaffold Accessibility for Cell Type Specific Invasion” (Version 2) [Dataset]. https://doi.org/10.17863/CAM.23496
Description
Zip folder containing sample reconstructed Micro-CT scans (.tif files) from each scaffold condition. Image pixel size is 3.1 μm. Each image plane is perpendicular to the direction of cell invasion (i.e. parallel to the seeding plane). Two Microsoft Excel files, containing the raw measurements of pore size and of L and d (as defined in the manuscript) for calculation of percolation diameter. Units are in pixels unless specified. Zip folder containing raw images (.png files) of each scaffold section, in bright field and in the green fluorescence channel showing actin staining. NB: Individual images are labelled according to a lab-based numbering scheme and do not match the sample nomenclature used in the manuscript. Microsoft Excel files for each cell type and time point showing the calculations of median cell position obtained from the images supplied, following the procedure outlined in the manuscript. The values of median cell position shown in black typeface were averaged to give the mean of six measurements. For each condition, these six measurements were chosen from replicates containing consistency in collagen pore wall orientation, while ensuring it was kept above OI = 0.9 (indicating high collagen orientation in the direction of cell invasion). See the main manuscript for more details.
Format
Image and tabular data viewers; image processing software
Keywords
invasion, cell-type, pore size, percolation diameter, Micro-CT, cellular sieving
Relationships
Related research output: https://doi.org/10.1002/adbi.201700257
Publication Reference: https://doi.org/10.1002/adbi.201700257
Identifiers
This record's DOI: https://doi.org/10.17863/CAM.23496
Rights
Attribution 4.0 International (CC BY 4.0)
Licence URL: https://creativecommons.org/licenses/by/4.0/
Statistics
Total file downloads (since January 2020). For more information on metrics see the
IRUS guide.
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