The RNA-binding protein PTBP1 is necessary for B cell selection in germinal centers.
Díaz-Muñoz, Manuel D
Coulson, Richard MR
Bell, Sarah E
Smith, Christopher WJ
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Monzón-Casanova, E., Screen, M., Díaz-Muñoz, M. D., Coulson, R. M., Bell, S. E., Lamers, G., Solimena, M., et al. (2018). The RNA-binding protein PTBP1 is necessary for B cell selection in germinal centers.. Nature Immunology, 19 (3), 267-278. https://doi.org/10.1038/s41590-017-0035-5
Antibody affinity maturation occurs in germinal centers (GCs), where B cells cycle between the light zone (LZ) and the dark zone. In the LZ, GC B cells bearing immunoglobulins with the highest affinity for antigen receive positive selection signals from helper T cells, which promotes their rapid proliferation. Here we found that the RNA-binding protein PTBP1 was needed for the progression of GC B cells through late S phase of the cell cycle and for affinity maturation. PTBP1 was required for proper expression of the c-MYC-dependent gene program induced in GC B cells receiving T cell help and directly regulated the alternative splicing and abundance of transcripts that are increased during positive selection to promote proliferation.
Biotechnology and Biological Sciences Research Council (BB/J001457/1)
External DOI: https://doi.org/10.1038/s41590-017-0035-5
This record's URL: https://www.repository.cam.ac.uk/handle/1810/279041