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dc.contributor.authorGouil, Quentinen
dc.contributor.authorBaulcombe, Daviden
dc.date.accessioned2018-09-08T06:28:25Z
dc.date.available2018-09-08T06:28:25Z
dc.date.issued2018-03-20en
dc.identifier.issn1471-2164
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/279726
dc.description.abstractFreakish and rare or the tip of the iceberg? Both phrases have been used to refer to paramutation, an epigenetic drive that contravenes Mendel’s first law of segregation. Although its underlying mechanisms are beginning to unravel, its under-standing relies only on a few examples that may involve trans-genes or artificially generated epialleles. By using DNA methy-lation of introgression lines as an indication of past paramu-tation, we reveal that the paramutation-like properties of the H06 locus in hybrids of Solanum lycopersicum and a range of tomato relatives and cultivars depend on the timing of sRNA production and conform to an RNA-directed mechanism. In addition, by scanning the methylomes of tomato introgression lines for shared regions of differential methylation that are ab-sent in the S. lycopersicum parent, we identify thousands of can-didate regions for paramutation-like behaviour. The methyla-tion patterns for a subset of these regions segregate with non Mendelian ratios, consistent with secondary paramutation-like interactions to variable extents depending on the locus. To-gether these results demonstrate that paramutation-like epige-netic interactions are common for natural epialleles in tomato, but vary in timing and penetrance. epigenetics | paramutation | DNA methylation | small RNAs | RNA-directed DNA methylation | hybrids | introgression lines
dc.format.mediumElectronicen
dc.languageengen
dc.publisherBMC Genomics
dc.rightsAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectLycopersicon esculentumen
dc.subjectRNA, Small Interferingen
dc.subjectDNA Methylationen
dc.subjectEpigenesis, Geneticen
dc.subjectGene Silencingen
dc.subjectMutationen
dc.subjectAllelesen
dc.subjectTransgenesen
dc.titleParamutation-like features of multiple natural epialleles in tomato.en
dc.typeArticle
prism.issueIdentifier1en
prism.publicationDate2018en
prism.publicationNameBMC genomicsen
prism.startingPage203
prism.volume19en
dc.identifier.doi10.17863/CAM.27097
dcterms.dateAccepted2018-03-09en
rioxxterms.versionofrecord10.1186/s12864-018-4590-4en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2018-03-20en
dc.contributor.orcidGouil, Quentin [0000-0002-5142-7886]
dc.contributor.orcidBaulcombe, David [0000-0003-0780-6878]
dc.identifier.eissn1471-2164
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idEuropean Research Council (340642)


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)