Show simple item record

dc.contributor.authorSangineto, Moris
dc.contributor.authorGraziano, Giusi
dc.contributor.authorD'Amore, Simona
dc.contributor.authorSalvia, Roberto
dc.contributor.authorPalasciano, Giuseppe
dc.contributor.authorSabbà, Carlo
dc.contributor.authorVacca, Michele
dc.contributor.authorCariello, Marica
dc.date.accessioned2018-09-20T12:07:49Z
dc.date.available2018-09-20T12:07:49Z
dc.date.issued2018
dc.identifier.issn1932-6203
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/280570
dc.description.abstractCeliac disease (CD) is a systemic disorder characterized by an immune-mediated reaction to gluten and a wide spectrum of clinical manifestations. Currently, the main treatment of CD is represented by adherence to a gluten-free diet (GFD) which determines the resolution of symptoms, and the normalization of the serology and of the duodenal villous atrophy. In the present study, we aimed to identify changes in gene expression in peripheral blood mononuclear cells (PBMCs) of celiac patients on GFD for at least 2 years, in order to identify novel disease biomarkers and candidate targets for putative therapeutic approaches. Microarray analysis was performed on PBMCs from 17 celiac patients on long-term GFD and 20 healthy controls. We identified 517 annotated genes that were significantly modulated between celiac patients and controls. Significant biological pathways were functionally clustered using the Core Function of Ingenuity System Pathway Analysis (IPA). Intriguingly, despite being on a GFD, celiac patients exhibited a peculiar PBMC profile characterized by an aberrant expression of genes involved in the regulation of immunity, inflammatory response, metabolism, and cell proliferation. Random forest algorithm was then used to validate the prediction ability of core genes as classifiers of the "celiac status". In conclusion, our study identified a characteristic PBMCs signature profile in clinically asymptomatic celiac patient.
dc.format.mediumElectronic-eCollection
dc.languageeng
dc.publisherPublic Library of Science (PLoS)
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectLeukocytes, Mononuclear
dc.subjectHumans
dc.subjectCeliac Disease
dc.subjectMicroarray Analysis
dc.subjectGene Expression Profiling
dc.subjectGene Expression Regulation
dc.subjectAdult
dc.subjectFemale
dc.subjectMale
dc.subjectDiet, Gluten-Free
dc.subjectTranscriptome
dc.titleIdentification of peculiar gene expression profile in peripheral blood mononuclear cells (PBMC) of celiac patients on gluten free diet.
dc.typeArticle
prism.issueIdentifier5
prism.publicationDate2018
prism.publicationNamePLoS One
prism.startingPagee0197915
prism.volume13
dc.identifier.doi10.17863/CAM.27938
dcterms.dateAccepted2018-05-10
rioxxterms.versionofrecord10.1371/journal.pone.0197915
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-01
dc.contributor.orcidCariello, Marica [0000-0002-7537-6657]
dc.identifier.eissn1932-6203
rioxxterms.typeJournal Article/Review
cam.issuedOnline2018-05-24


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International