Radiolabelled leucocytes in human pulmonary disease.
Solanki, Chandra K
Peters, A Michael
British medical bulletin
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Farahi, N., Loutsios, C., Tregay, N., Summers, C., Lok, L., Ruparelia, P., Solanki, C. K., et al. (2018). Radiolabelled leucocytes in human pulmonary disease.. British medical bulletin, 127 (1), 69-82. https://doi.org/10.1093/bmb/ldy022
The early blood cell labels, P-32-labelled di-isopropylfluorophosphate (DFP-32) and H-3, are non-gamma emitters and cannot be imaged, so early work with radiolabelled leucocytes was limited to kinetic studies based on counting radioactivity in sampled blood. These studies, nevertheless, were seminal and established the residence time of granulocytes in blood and their distribution between circulating and marginating pools. Cr-51 labelling was a step towards imaging but, because this radionuclide is an inefficient emitter of gamma photons, moreover at too high an energy for imaging, only surface counting over organs with scintillation probes could be used to determine the in vivo distribution of labelled cells. In the mid-seventies, Matthew Thakur, a radiochemist at Hammersmith Hospital, and John McAfee, a visiting nuclear medicine physician from USA, achieved their aim, within McAfee’s 6 month sabbatical, of labelling blood cells, including leucocytes, with the efficient gamma emitter, In-111, thereby allowing imaging of infection and inflammation and determination of granulocyte kinetics. It was several years later before Tc-99m labelling was developed to complement In-111 labelling. Lymphocytes, however, remained problematic because of their exquisite radiosensitivity.
Leukocytes, Granulocytes, Humans, Lung Diseases, Radioisotopes, Positron-Emission Tomography, Isotope Labeling
Asthma UK, NIHR Cambridge Biomedical Research Centre, Royal Papworth Hospital, AstraZeneca, GlaxoSmithKline
Asthma UK (05/066)
Cambridge University Hospitals NHS Foundation Trust (CUH) (unknown)
Addenbrooke's Charitable Trust (ACT) (mINUTE 14/16 C (IV))
Cambridge University Hospitals NHS Foundation Trust (CUH) (146281)
External DOI: https://doi.org/10.1093/bmb/ldy022
This record's URL: https://www.repository.cam.ac.uk/handle/1810/282818