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dc.contributor.authorKrogager, Toke P
dc.contributor.authorErnst, Russell J
dc.contributor.authorElliott, Thomas S
dc.contributor.authorCalo, Laura
dc.contributor.authorBeránek, Václav
dc.contributor.authorCiabatti, Ernesto
dc.contributor.authorSpillantini, Maria
dc.contributor.authorTripodi, Marco
dc.contributor.authorHastings, Michael
dc.contributor.authorChin, Jason
dc.date.accessioned2018-10-09T09:06:51Z
dc.date.available2018-10-09T09:06:51Z
dc.date.issued2018-02
dc.identifier.issn1087-0156
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/283245
dc.description.abstractWe develop an approach to tag proteomes synthesized by specific cell types in dissociated cortex, brain slices, and the brains of live mice. By viral-mediated expression of an orthogonal pyrrolysyl-tRNA synthetase-tRNAXXX pair in a cell type of interest and providing a non-canonical amino acid with a chemical handle, we selectively label neuronal or glial proteomes. The method enables the identification of proteins from spatially and genetically defined regions of the brain.
dc.description.sponsorshipMichael J Fox Foundation (MJFF) (unknown), Parkinson's UK (None), Cure Parkinson's Trust (unknown)
dc.languageeng
dc.publisherSpringer Nature
dc.titleLabeling and identifying cell-specific proteomes in the mouse brain.
dc.typeArticle
prism.endingPage159
prism.issueIdentifier2
prism.publicationDate2018
prism.publicationNameNature Biotechnology
prism.startingPage156
prism.volume36
dc.identifier.doi10.17863/CAM.30610
dcterms.dateAccepted2017-12-08
rioxxterms.versionofrecord10.1038/nbt.4056
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-02
dc.contributor.orcidSpillantini, Maria [0000-0002-8544-7332]
dc.contributor.orcidHastings, Michael [0000-0001-8576-6651]
dc.contributor.orcidChin, Jason [0000-0003-1219-4757]
dc.identifier.eissn1546-1696
dc.publisher.urlhttps://www.nature.com/articles/nbt.4056
rioxxterms.typeJournal Article/Review
pubs.funder-project-idMichael J Fox Foundation (MJFF) (unknown)
pubs.funder-project-idParkinson's UK (None)
pubs.funder-project-idCure Parkinson's Trust (unknown)
cam.issuedOnline2017-12-18
dc.identifier.urlhttps://www.nature.com/articles/nbt.4056


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