Citrate bridges between mineral platelets in bone.
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Authors
Davies, Erika
Müller, Karin H
Wong, Wai Ching
Pickard, Chris J
Reid, David G
Skepper, Jeremy N
Duer, Melinda J
Publication Date
2014-04-08Journal Title
Proceedings of the National Academy of Sciences of the United States of America
ISSN
1091-6490
Publisher
National Academy of Sciences
Volume
111
Issue
14
Pages
E1354-E1363
Language
eng
Type
Article
Metadata
Show full item recordCitation
Davies, E., Müller, K. H., Wong, W. C., Pickard, C. J., Reid, D. G., Skepper, J. N., & Duer, M. J. (2014). Citrate bridges between mineral platelets in bone.. Proceedings of the National Academy of Sciences of the United States of America, 111 (14), E1354-E1363. https://doi.org/10.1073/pnas.1315080111
Abstract
We provide evidence that citrate anions bridge between mineral platelets in bone and hypothesize that their presence acts to maintain separate platelets with disordered regions between them rather than gradual transformations into larger, more ordered blocks of mineral. To assess this hypothesis, we take as a model for a citrate bridging between layers of calcium phosphate mineral a double salt octacalcium phosphate citrate (OCP-citrate). We use a combination of multinuclear solid-state NMR spectroscopy, powder X-ray diffraction, and first principles electronic structure calculations to propose a quantitative structure for this material, in which citrate anions reside in a hydrated layer, bridging between apatitic layers. To assess the relevance of such a structure in native bone mineral, we present for the first time, to our knowledge, (17)O NMR data on bone and compare them with (17)O NMR data for OCP-citrate and other calcium phosphate minerals relevant to bone. The proposed structural model that we deduce from this work for bone mineral is a layered structure with thin apatitic platelets sandwiched between OCP-citrate-like hydrated layers. Such a structure can explain a number of known structural features of bone mineral: the thin, plate-like morphology of mature bone mineral crystals, the presence of significant quantities of strongly bound water molecules, and the relatively high concentration of hydrogen phosphate as well as the maintenance of a disordered region between mineral platelets.
Keywords
NMR crystallography, biomineralization, Animals, Bone and Bones, Calcium Phosphates, Citric Acid, Horses, Magnetic Resonance Spectroscopy, Minerals, Powder Diffraction, Rabbits
Sponsorship
Engineering and Physical Sciences Research Council (EP/F022778/1)
Identifiers
External DOI: https://doi.org/10.1073/pnas.1315080111
This record's URL: https://www.repository.cam.ac.uk/handle/1810/284418
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http://www.rioxx.net/licenses/all-rights-reserved
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