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dc.contributor.authorJensen, Jacob Krüger
dc.contributor.authorBusse-Wicher, Marta
dc.contributor.authorPoulsen, Christian Peter
dc.contributor.authorFangel, Jonatan Ulrik
dc.contributor.authorSmith, Peter James
dc.contributor.authorYang, Jeong-Yeh
dc.contributor.authorPeña, Maria-Jesus
dc.contributor.authorDinesen, Malene Hessellund
dc.contributor.authorMartens, Helle Juel
dc.contributor.authorMelkonian, Michael
dc.contributor.authorWong, Gane Ka-Shu
dc.contributor.authorMoremen, Kelley W
dc.contributor.authorWilkerson, Curtis Gene
dc.contributor.authorScheller, Henrik Vibe
dc.contributor.authorDupree, Paul
dc.contributor.authorUlvskov, Peter
dc.contributor.authorUrbanowicz, Breeanna Rae
dc.contributor.authorHarholt, Jesper
dc.date.accessioned2018-11-05T10:25:49Z
dc.date.available2018-11-05T10:25:49Z
dc.date.issued2018-05
dc.identifier.issn0028-646X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/284601
dc.description.abstractInsights into the evolution of plant cell walls have important implications for comprehending these diverse and abundant biological structures. In order to understand the evolving structure-function relationships of the plant cell wall, it is imperative to trace the origin of its different components. The present study is focused on plant 1,4-β-xylan, tracing its evolutionary origin by genome and transcriptome mining followed by phylogenetic analysis, utilizing a large selection of plants and algae. It substantiates the findings by heterologous expression and biochemical characterization of a charophyte alga xylan synthase. Of the 12 known gene classes involved in 1,4-β-xylan formation, XYS1/IRX10 in plants, IRX7, IRX8, IRX9, IRX14 and GUX occurred for the first time in charophyte algae. An XYS1/IRX10 ortholog from Klebsormidium flaccidum, designated K. flaccidumXYLAN SYNTHASE-1 (KfXYS1), possesses 1,4-β-xylan synthase activity, and 1,4-β-xylan occurs in the K. flaccidum cell wall. These data suggest that plant 1,4-β-xylan originated in charophytes and shed light on the origin of one of the key cell wall innovations to occur in charophyte algae, facilitating terrestrialization and emergence of polysaccharide-based plant cell walls.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherWiley
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCell Wall
dc.subjectHumans
dc.subjectPentosyltransferases
dc.subjectEvolution, Molecular
dc.subjectPhylogeny
dc.subjectAmino Acid Motifs
dc.subjectBiosynthetic Pathways
dc.subjectHEK293 Cells
dc.subjectCharophyceae
dc.subjectPlant Cells
dc.titleIdentification of an algal xylan synthase indicates that there is functional orthology between algal and plant cell wall biosynthesis.
dc.typeArticle
prism.endingPage1060
prism.issueIdentifier3
prism.publicationDate2018
prism.publicationNameNew Phytol
prism.startingPage1049
prism.volume218
dc.identifier.doi10.17863/CAM.31975
dcterms.dateAccepted2018-01-14
rioxxterms.versionofrecord10.1111/nph.15050
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-05
dc.contributor.orcidDupree, Paul [0000-0001-9270-6286]
dc.identifier.eissn1469-8137
rioxxterms.typeJournal Article/Review
pubs.funder-project-idLeverhulme Trust (RP2013-SL-008)
cam.issuedOnline2018-02-20


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International