Neuroprotection of retinal ganglion cells by a novel gene therapy construct that achieves sustained enhancement of brain-derived neurotrophic factor/tropomyosin-related kinase receptor-B signaling.
Khatib, Tasneem Z
Barber, Amanda C
Kong, George YX
Widdowson, Peter S
Cell death & disease
Nature Publishing Group
MetadataShow full item record
Osborne, A., Khatib, T. Z., Songra, L., Barber, A. C., Hall, K., Kong, G. Y., Widdowson, P. S., & et al. (2018). Neuroprotection of retinal ganglion cells by a novel gene therapy construct that achieves sustained enhancement of brain-derived neurotrophic factor/tropomyosin-related kinase receptor-B signaling.. Cell death & disease, 9 (10), 1007. https://doi.org/10.1038/s41419-018-1041-8
Previous studies have demonstrated that intravitreal delivery of brain-derived neurotrophic factor (BDNF) by injection of recombinant protein or by gene therapy can alleviate retinal ganglion cell (RGC) loss after optic nerve injury. BDNF gene therapy can improved RGC survival in experimental models of glaucoma, the leading cause of irreversible blindness worldwide. However, the therapeutic efficacy of BDNF supplementation alone is time limited due at least in part to BDNF receptor downregulation. Tropomyosin-related receptor kinase-B (TrkB) downregulation has been reported in many neurological diseases including glaucoma, potentially limiting the effect of sustained or repeated BDNF delivery. Here we characterize a novel adeno-associated virus (AAV) gene therapy (AAV2 TrkB-2A-mBDNF) that not only increases BDNF production but also improves long term neuroprotective signaling by increasing expression of the BDNF receptor (TrkB) within the inner retina. This approach leads to significant and sustained elevation of survival signaling pathways ERK and AKT within RGCs over 6 months and avoids the receptor downregulation which we observe with treatment with AAV2 BDNF alone. We validate the neuroprotective efficacy of AAV2 TrkB-2A-mBDNF in a mouse model of optic nerve injury, where it outperforms conventional AAV2 BDNF or AAV2 TrkB therapy, before showing powerful proof of concept neuroprotection of RGCs and axons in a rat model of chronic intraocular pressure (IOP) elevation. We also show that there are no adverse effects of the vector on retinal structure or function as assessed by histology and electroretinography in young or aged animals. Further studies are underway to explore the potential of this vector as a candidate for progression into clinical studies to protect RGCs in patients with glaucoma and progressive visual loss despite conventional IOP-lowering treatment.
Axons, Retinal Ganglion Cells, Retina, Animals, Mice, Inbred C57BL, Humans, Mice, Rats, Rats, Sprague-Dawley, Dependovirus, Optic Nerve Injuries, Glaucoma, Disease Models, Animal, Receptor, trkB, Brain-Derived Neurotrophic Factor, Membrane Glycoproteins, Signal Transduction, Down-Regulation, Intraocular Pressure, Male, HEK293 Cells, Genetic Therapy, Neuroprotection
This work was supported by the Wellcome Trust (Pathfinder Award), the Midven Rainbow Seed Fund, Quethera Ltd, University of Cambridge Enterprise, the HB Allen Charitable Trust and the Cambridge Eye Trust.
Cambridge Eye Trust (unknown)
External DOI: https://doi.org/10.1038/s41419-018-1041-8
This record's URL: https://www.repository.cam.ac.uk/handle/1810/285329
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/