Actomyosin-driven tension at compartmental boundaries orients cell division independently of cell geometry in vivo
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Publication Date
2018-12-17Journal Title
Developmental Cell
ISSN
1534-5807
Publisher
Cold Spring Harbor Laboratory
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Scarpa, E., Finet, C., Blanchard, G., & Sanson, B. (2018). Actomyosin-driven tension at compartmental boundaries orients cell division independently of cell geometry in vivo. Developmental Cell https://doi.org/10.1016/j.devcel.2018.10.029
Abstract
During animal development, planar polarization of the actomyosin cytoskeleton underlies key morphogenetic events such as axis extension and boundary formation. Actomyosin is enriched along compartment boundaries during segmentation of the Drosophila embryo, forming supracellular contractile cables that keep cells segregated at boundaries. Here, we show that these contractile actomyosin cables bias the orientation of division in cells in contact with compartment boundaries. By decreasing actomyosin cable tension locally using laser ablation or, conversely ectopically increasing tension using laser wounding, we demonstrate that localised subcellular force is necessary and sufficient to orient mitoses in vivo. Moreover this bias is independent of cell geometry and involves capture of the spindle pole by the actomyosin cortex.
Sponsorship
Wellcome Trust (099234/Z/12/Z)
Biotechnology and Biological Sciences Research Council (BB/J010278/1)
Wellcome Trust (207553/Z/17/Z)
Identifiers
External DOI: https://doi.org/10.1016/j.devcel.2018.10.029
This record's URL: https://www.repository.cam.ac.uk/handle/1810/286733
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