Suppressor of cytokine signalling protein SOCS3 expression is increased at sites of acute and chronic inflammation.
View / Open Files
Authors
White, Gemma E
Cotterill, Andrew
Addley, Mark R
Soilleux, Elizabeth J
Greaves, David R
Publication Date
2011-04Journal Title
J Mol Histol
ISSN
1567-2379
Publisher
Springer Science and Business Media LLC
Volume
42
Issue
2
Pages
137-151
Language
eng
Type
Article
This Version
VoR
Physical Medium
Print-Electronic
Metadata
Show full item recordCitation
White, G. E., Cotterill, A., Addley, M. R., Soilleux, E. J., & Greaves, D. R. (2011). Suppressor of cytokine signalling protein SOCS3 expression is increased at sites of acute and chronic inflammation.. J Mol Histol, 42 (2), 137-151. https://doi.org/10.1007/s10735-011-9317-7
Abstract
Treatment of cells with cytokines and growth factors leads to the synthesis of Suppressor of Cytokine Signalling (SOCS) proteins that act as potent negative regulators of signalling via the Jak/STAT pathway. We used immunohistochemistry to identify cells and pathologies where SOCS3 expression might influence acute and chronic inflammatory responses in human tissues. Epitope and GFP tagged SOCS3 fusion proteins were localised predominantly in the nucleus of transfected cells and a validated anti SOCS3 antiserum revealed the expression of SOCS3 in the nucleus and cytoplasm of macrophages, endothelial and epithelial cells in a wide range of normal tissues in tissue microarrays (n = 31 different tissues). Nuclear SOCS3 was only seen in cells expressing a high level of the protein. Comparative immunostaining of acute, chronically and granulomatously inflamed human tissues revealed higher levels of nuclear and cytoplasmic SOCS3 expression in inflamed than in corresponding normal tissues, particularly in recruited leukocyte populations, but also in epithelia. The staining appeared more intense, suggesting higher expression levels, in areas where inflammation was more acute, consistent with the time course of SOCS3 induction described in vitro. Expression of SOCS3 protein by leucocytes and other cell types in tissue sections could be a useful marker of cells undergoing acute or chronic stimulation by cytokines in vivo.
Keywords
CHO Cells, Animals, Humans, Cricetulus, Appendicitis, Inflammatory Bowel Diseases, Sarcoidosis, Acute-Phase Reaction, beta-Galactosidase, Luciferases, Organ Specificity, Gene Expression, Up-Regulation, Protein Transport, Genes, Reporter, Female, Cricetinae, Male, Suppressor of Cytokine Signaling Proteins, Giant Cell Arteritis, HEK293 Cells, Biomarkers, Suppressor of Cytokine Signaling 3 Protein
Identifiers
External DOI: https://doi.org/10.1007/s10735-011-9317-7
This record's URL: https://www.repository.cam.ac.uk/handle/1810/287406
Statistics
Total file downloads (since January 2020). For more information on metrics see the
IRUS guide.
Recommended or similar items
The current recommendation prototype on the Apollo Repository will be turned off on 03 February 2023. Although the pilot has been fruitful for both parties, the service provider IKVA is focusing on horizon scanning products and so the recommender service can no longer be supported. We recognise the importance of recommender services in supporting research discovery and are evaluating offerings from other service providers. If you would like to offer feedback on this decision please contact us on: support@repository.cam.ac.uk