One-pot stapling of interchain disulfides of antibodies using an isobutylene motif.
Marques, Marta C
Torres, Vukosava M
Lopes Bernardes, Goncalo
Organic & biomolecular chemistry
Royal Society of Chemistry (RSC)
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Sun, S., Akkapeddi, P., Marques, M. C., Martínez-Sáez, N., Torres, V. M., Cordeiro, C., Boutureira, O., & et al. (2019). One-pot stapling of interchain disulfides of antibodies using an isobutylene motif.. Organic & biomolecular chemistry, 17 (7), 2005-2012. https://doi.org/10.1039/c8ob02877j
Monoclonal antibodies have emerged as an important class of therapeutics in oncological and autoimmune diseases due to several attractive properties, such as high binding affinity and specificity. However, it has recently become clear that antibodies recovered from serum show significantly decreased potency owing to various reasons, including deamidation, oxidation, fragment antigen binding (Fab) exchange, and disulfide shuffling. Fab exchange and disulfide shuffling result because of the instability of disulfides in serum. Herein, we reported a ‘one-pot’ stapling strategy using isobutylene motifs to stabilise the interchain disulfides of antibodies. This general method was applied to a Fab fragment of anti-HER2 antibody. The stapled Fab was completely stable in the presence of biological thiols. The approach was further applied to two different full-length IgG, trastuzumab and rituximab, under mild and biocompatible conditions. The binding affinity of the antibody was enhanced, relative to its native form, after being stapled. The stapled structure maintained its effectors functions and behaved similarly as its native form in vivo. This work provides a straightforward and scalable method for the stabilisation antibodies in various formats.
Royal Society (uf110046)
European Commission Horizon 2020 (H2020) ERC (676832)
Royal Society (URF\R\180019)
European Commission Horizon 2020 (H2020) Marie Sk?odowska-Curie actions (675007)
External DOI: https://doi.org/10.1039/c8ob02877j
This record's URL: https://www.repository.cam.ac.uk/handle/1810/287420