Show simple item record

dc.contributor.authorBaillie-Johnson, Peteren
dc.contributor.authorVoiculescu, Octavianen
dc.contributor.authorHayward, Pennyen
dc.contributor.authorSteventon, Benjaminen
dc.date.accessioned2019-01-16T00:31:10Z
dc.date.available2019-01-16T00:31:10Z
dc.date.issued2018-01en
dc.identifier.issn1422-6405
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/288039
dc.description.abstractNeuromesodermal progenitors (NMps) are a population of bipotent progenitors that maintain competence to generate both spinal cord and paraxial mesoderm throughout the elongation of the posterior body axis. Recent studies have generated populations of NMp-like cells in culture, which have been shown to differentiate to both neural and mesodermal cell fates when transplanted into either mouse or chick embryos. Here, we aim to compare the potential of mouse embryonic stem (ES) cell-derived progenitor populations to generate NMp behaviour against both undifferentiated and differentiated populations. We define NMp behaviour as the ability of cells to i) contribute to a significant proportion of the anterior-posterior body axis, ii) enter into both posterior neural and somitic compartments and, iii) retain a proportion of the progenitor population within the posterior growth zone. We compare previously identified ES cell-derived NMp-like populations to undifferentiated mouse ES cells and find that they all display similar potentials to generate NMp behaviour in vivo. To assess whether this competence is lost upon further differentiation, we generated anterior and posterior embryonic cell types through the generation of 3D gastruloids and show that NMp competence is lost within the anterior (Brachyury negative) portion of the gastruloid. Together this suggests that in vitro derived NMp-like cells maintain an ability to contribute to multiple germ layers that is also present within pluripotent ES cells, rather than acquiring a neuromesodermal competent state through differentiation.
dc.description.sponsorshipWellcome Trust (109408) and (RCDF 088380/09/Z) Engineering and Physical Sciences Research Council (EPSRC) Studentship (1359454).
dc.format.mediumPrint-Electronicen
dc.languageengen
dc.publisherKarger
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectSpinal Corden
dc.subjectCell Lineen
dc.subjectChick Embryoen
dc.subjectGerm Layersen
dc.subjectMesodermen
dc.subjectAnimalsen
dc.subjectMiceen
dc.subjectEmbryo Culture Techniquesen
dc.subjectCell Differentiationen
dc.subjectCell Lineageen
dc.subjectBody Patterningen
dc.subjectNeural Stem Cellsen
dc.subjectMouse Embryonic Stem Cellsen
dc.titleThe Chick Caudolateral Epiblast Acts as a Permissive Niche for Generating Neuromesodermal Progenitor Behaviours.en
dc.typeArticle
prism.endingPage330
prism.issueIdentifier5-6en
prism.publicationDate2018en
prism.publicationNameCells, tissues, organsen
prism.startingPage320
prism.volume205en
dc.identifier.doi10.17863/CAM.35358
dcterms.dateAccepted2018-10-18en
rioxxterms.versionofrecord10.1159/000494769en
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2018-01en
dc.contributor.orcidBaillie-Johnson, Peter [0000-0003-2157-5017]
dc.contributor.orcidVoiculescu, Octavian [0000-0003-0714-1482]
dc.contributor.orcidSteventon, Benjamin [0000-0001-7838-839X]
dc.identifier.eissn1422-6421
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWELLCOME TRUST (109408/Z/15/Z)
pubs.funder-project-idWellcome Trust (088380/Z/09/Z)


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's licence is described as Attribution-NonCommercial-NoDerivatives 4.0 International