Analysis of the Localization of MEN Components by Live Cell Imaging Microscopy.
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Publication Date
2017ISBN
9781493965007
Publisher
Springer New York
Volume
1505
Pages
151-166
Language
eng
Type
Book chapter
This Version
AM
Physical Medium
Print
Metadata
Show full item recordCitation
Guo, Z., & Segal, M. (2017). Analysis of the Localization of MEN Components by Live Cell Imaging Microscopy.. [Book chapter]. https://doi.org/10.1007/978-1-4939-6502-1_12
Abstract
Mitotic exit is determined by multiple spatial and temporal cues from the spindle poles and the two compartments in a dividing yeast cell-the mother and the bud. These signals are ultimately integrated by the activation of the mitotic exit network (MEN) to promote persistent release of Cdc14 from the nucleolus. Live imaging analysis using fluorescent protein tags is invaluable to dissect this critical decision-making trigger. Here, we present protocols for routine yeast live cell microscopy applicable to this problem.
Keywords
Cell population analysis, Digital image, Fluorescence microscopy, Fluorescent protein tags, Live cell imaging, Single cell analysis, Still imaging, Time lapse, Anaphase, Cell Cycle Proteins, Green Fluorescent Proteins, Image Processing, Computer-Assisted, Microscopy, Fluorescence, Mitosis, Optical Imaging, Protein Serine-Threonine Kinases, Protein Tyrosine Phosphatases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Single-Cell Analysis
Identifiers
External DOI: https://doi.org/10.1007/978-1-4939-6502-1_12
This record's DOI: https://doi.org/10.17863/CAM.38790
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