Analysis of the Localization of MEN Components by Live Cell Imaging Microscopy.
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Guo, Z., & Segal, M. (2017). Analysis of the Localization of MEN Components by Live Cell Imaging Microscopy.. [Book chapter]. https://doi.org/10.1007/978-1-4939-6502-1_12
Mitotic exit is determined by multiple spatial and temporal cues from the spindle poles and the two compartments in a dividing yeast cell-the mother and the bud. These signals are ultimately integrated by the activation of the mitotic exit network (MEN) to promote persistent release of Cdc14 from the nucleolus. Live imaging analysis using fluorescent protein tags is invaluable to dissect this critical decision-making trigger. Here, we present protocols for routine yeast live cell microscopy applicable to this problem.
Saccharomyces cerevisiae, Protein-Serine-Threonine Kinases, Cell Cycle Proteins, Saccharomyces cerevisiae Proteins, Green Fluorescent Proteins, Microscopy, Fluorescence, Anaphase, Mitosis, Image Processing, Computer-Assisted, Protein Tyrosine Phosphatases, Single-Cell Analysis, Optical Imaging
External DOI: https://doi.org/10.1007/978-1-4939-6502-1_12
This record's DOI: https://doi.org/10.17863/CAM.38790
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