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A patient-derived explant (PDE) model of hormone-dependent cancer.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Centenera, Margaret M 
Hickey, Theresa E 
Jindal, Shalini 
Ryan, Natalie K 
Ravindranathan, Preethi 

Abstract

Breast and prostate cancer research to date has largely been predicated on the use of cell lines in vitro or in vivo. These limitations have led to the development of more clinically relevant models, such as organoids or murine xenografts that utilize patient-derived material; however, issues related to low take rate, long duration of establishment, and the associated costs constrain use of these models. This study demonstrates that ex vivo culture of freshly resected breast and prostate tumor specimens obtained from surgery, termed patient-derived explants (PDEs), provides a high-throughput and cost-effective model that retains the native tissue architecture, microenvironment, cell viability, and key oncogenic drivers. The PDE model provides a unique approach for direct evaluation of drug responses on an individual patient's tumor, which is amenable to analysis using contemporary genomic technologies. The ability to rapidly evaluate drug efficacy in patient-derived material has high potential to facilitate implementation of personalized medicine approaches.

Description

Keywords

ex vivo culture, patient-derived explant, preclinical tumor model

Journal Title

Molecular Oncology

Conference Name

Journal ISSN

1878-0261
1878-0261

Volume Title

12

Publisher

John Wiley and Sons Inc.
Sponsorship
Cancer Research UK (C14303/A17197)
Cancer Research UK (20411)
Cancer Research UK and ERC.