Epistatic interactions can moderate the antigenic effect of substitutions in haemagglutinin of influenza H3N2 virus.
Accepted version
Peer-reviewed
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Authors
Koel, Björn F
Burke, David F
van der Vliet, Stefan
Bestebroer, Theo M
Rimmelzwaan, Guus F
Abstract
We previously showed that single amino acid substitutions at seven positions in haemagglutinin determined major antigenic change of influenza H3N2 virus. Here, the impact of two such substitutions was tested in 11 representative H3 haemagglutinins to investigate context-dependence effects. The antigenic effect of substitutions introduced at haemagglutinin position 145 was fully independent of the amino acid context of the representative haemagglutinins. Antigenic change caused by substitutions introduced at haemagglutinin position 155 was variable and context-dependent. Our results suggest that epistatic interactions with contextual amino acids in the haemagglutinin can moderate the magnitude of antigenic change.
Description
Keywords
antigenic evolution, epistasis, influenza virus, Amino Acid Substitution, Antigens, Viral, Epistasis, Genetic, Hemagglutinin Glycoproteins, Influenza Virus, Humans, Influenza A Virus, H3N2 Subtype, Influenza, Human, Mutant Proteins
Journal Title
J Gen Virol
Conference Name
Journal ISSN
0022-1317
1465-2099
1465-2099
Volume Title
100
Publisher
Microbiology Society
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All rights reserved
Sponsorship
Office of the Director (DP1OD000490)
Human Frontier Science Program (HFSP) (RG P0050/2008-C-SMITH)
European Commission (223498)
European Commission (278976)
National Institutes of Health (NIH) (via Mount Sinai School of Medicine (MSSM)) (#HHSN2662007000010C)
National Institutes of Health (NIH) (via Mount Sinai School of Medicine (MSSM)) (0258-0513/HHSN272201400008C)
National Institutes of Health (NIH) (via Mount Sinai School of Medicine (MSSM)) (HHSN272201400008C)
National Institutes of Health (NIH) (via Mount Sinai School of Medicine (MSSM)) (HHSN272201400008C)
National Institutes of Health (NIH) (via Mount Sinai School of Medicine (MSSM)) (HHSN272201400008C - Option 20B)
National Institutes of Health (NIH) (via Mount Sinai School of Medicine (MSSM)) (HHSN272201400008C)
Human Frontier Science Program (HFSP) (RG P0050/2008-C-SMITH)
European Commission (223498)
European Commission (278976)
National Institutes of Health (NIH) (via Mount Sinai School of Medicine (MSSM)) (#HHSN2662007000010C)
National Institutes of Health (NIH) (via Mount Sinai School of Medicine (MSSM)) (0258-0513/HHSN272201400008C)
National Institutes of Health (NIH) (via Mount Sinai School of Medicine (MSSM)) (HHSN272201400008C)
National Institutes of Health (NIH) (via Mount Sinai School of Medicine (MSSM)) (HHSN272201400008C)
National Institutes of Health (NIH) (via Mount Sinai School of Medicine (MSSM)) (HHSN272201400008C - Option 20B)
National Institutes of Health (NIH) (via Mount Sinai School of Medicine (MSSM)) (HHSN272201400008C)