Application of single-cell RNA sequencing methodologies in understanding haematopoiesis and immunology.
Essays in biochemistry
Portland Press, Ltd.
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Ranzoni, A., Strzelecka, P., & Cvejic, A. (2019). Application of single-cell RNA sequencing methodologies in understanding haematopoiesis and immunology.. Essays in biochemistry, 63 (2), 217-225. https://doi.org/10.1042/ebc20180072
The blood and immune system is characterised by utmost diversity in its cellular components. This heterogeneity can solely be resolved with the application of single cell technologies that enable precise examination of cell-to-cell variation. Single-cell transcriptomics is continuously pushing forward our understanding of processes driving haematopoiesis and immune responses in physiological settings as well as in disease. Remarkably, in the last five years, a number of studies involving single-cell RNA sequencing allowed the discovery of new immune cell types and revealed that haematopoiesis is a continuous rather than a stepwise process, thus challenging the classical haematopoietic lineage tree model. This review summarises the most recent studies which applied single-cell RNA sequencing to answer outstanding questions in the fields of haematology and immunology and discusses the present challenges and future directions.
Cells, Cultured, Immune System, Animals, Humans, Mice, RNA, Small Cytoplasmic, Sequence Analysis, RNA, Hematopoiesis, Single-Cell Analysis
The study was supported by Cancer Research UK grant number C45041/A14953 and European Research Council project 677501 – ZF_Blood
Cancer Research UK (14953)
ECH2020 EUROPEAN RESEARCH COUNCIL (ERC) (677501)
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External DOI: https://doi.org/10.1042/ebc20180072
This record's URL: https://www.repository.cam.ac.uk/handle/1810/293023
All rights reserved, Attribution 4.0 International
Licence URL: http://creativecommons.org/licenses/by/4.0/