Standards for plant synthetic biology: a common syntax for exchange of DNA parts.
Patron, Nicola J
Jones, Jonathan DG
Van Esse, H Peter
Brutnell, Thomas P
Quick, W Paul
Fraser, Paul D
South, Paul F
Hamberger, Björn R
Murray, James AH
Edwards, Keith J
Blackwell Publishing Inc.
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Patron, N. J., Orzaez, D., Marillonnet, S., Warzecha, H., Matthewman, C., Youles, M., Raitskin, O., et al. (2015). Standards for plant synthetic biology: a common syntax for exchange of DNA parts.. New Phytologist, 208 (1), 13-19. https://doi.org/10.1111/nph.13532
Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.
DNA assembly, GoldenGate, Type IIS restriction endonucleases, cloning, genetic syntax, synthetic biology, Botany, Cloning, Molecular, DNA, Deoxyribonucleases, Type II Site-Specific, Eukaryota, Genetic Engineering, Plants, Plants, Genetically Modified, Plasmids, Reference Standards, Synthetic Biology, Transcription, Genetic
Biotechnological and Biological Sciences Research Council (BBSRC). Grant Numbers: BB/K005952/1, BB/L02182X/1 Synthetic Biology Research Centre ‘OpenPlant’ award. Grant Number: BB/L014130/1 Spanish MINECO. Grant Number: BIO2013‐42193‐R Engineering Nitrogen Symbiosis for Africa (ENSA) The Bill & Melinda Gates Foundation US Department of Energy, Office of Biological and Environmental. Grant Number: DE‐AC02‐05CH1123 COST Action. Grant Number: FA1006
BBSRC (via University College London (UCL)) (FCAGF)
Biotechnology and Biological Sciences Research Council (BB/L002957/1)
External DOI: https://doi.org/10.1111/nph.13532
This record's URL: https://www.repository.cam.ac.uk/handle/1810/293594