Repository logo
 

Structural Analysis using SHALiPE to Reveal RNA G‐Quadruplex Formation in Human Precursor MicroRNA

Published version
Peer-reviewed

Type

Article

Change log

Authors

Kwok, Chun Kit 
Sahakyan, Aleksandr B 
Balasubramanian, Shankar  ORCID logo  https://orcid.org/0000-0002-0281-5815

Abstract

jats:titleAbstract</jats:title>jats:pRNA G‐quadruplex (rG4) structures are of fundamental importance to biology. A novel approach is introduced to detect and structurally map rG4s at single‐nucleotide resolution in RNAs. The approach, denoted SHALiPE, couples selective 2′‐hydroxyl acylation with lithium ion‐based primer extension, and identifies characteristic structural fingerprints for rG4 mapping. We apply SHALiPE to interrogate the human precursor microRNA 149, and reveal the formation of an rG4 structure in this non‐coding RNA. Additional analyses support the SHALiPE results and uncover that this rG4 has a parallel topology, is thermally stable, and is conserved in mammals. An in vitro Dicer assay shows that this rG4 inhibits Dicer processing, supporting the potential role of rG4 structures in microRNA maturation and post‐transcriptional regulation of mRNAs.</jats:p>

Description

Keywords

31 Biological Sciences, 3102 Bioinformatics and Computational Biology, 3404 Medicinal and Biomolecular Chemistry, 34 Chemical Sciences, Genetics, Biotechnology, 2.1 Biological and endogenous factors

Journal Title

Angewandte Chemie

Conference Name

Journal ISSN

0044-8249
1521-3757

Volume Title

128

Publisher

Wiley
Sponsorship
European Research Council (339778)
Cancer Research Uk (None)