Evaluation of the Idexx ProCyte Dx® for analysis of canine cerebrospinal fluid.
The Journal of small animal practice
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Romero-Fernandez, N., Ives, E., Fraser, A., Williams, T., & Paul, A. (2020). Evaluation of the Idexx ProCyte Dx® for analysis of canine cerebrospinal fluid.. The Journal of small animal practice, 61 (2), 110-115. https://doi.org/10.1111/jsap.13088
OBJECTIVES To assess the utility of the Idexx Procyte Dx® haematology analyser for assessing total nucleated cell count (TNCC) and differential cell counts in canine cerebrospinal fluid (CSF). METHODS Seventy-three client-owned dogs undergoing investigations for pyrexia and/or neurological signs were prospectively included. Cerebrospinal fluid samples were assessed using an Idexx Procyte Dx® analyser and the results were compared to those obtained with the external laboratory reference standard. RESULTS The Idexx Procyte Dx® performed with good sensitivity (92.6%) and moderate specificity (67.4%) for TNCC when compared to the reference standard. Qualitative assessment of the Idexx ProCyte Dx® scatter plots, and quantitative assessment of differential cell counts when available, appeared to correlate well with the external laboratory manual differential cell counts, with a good-to-high agreement in 25/26 samples (96.2%). CONCLUSIONS AND CLINICAL RELEVANCE The Idexx Procyte Dx® analyser performed well in determining the TNCC and differential cell counts in canine CSF when compared to a reference standard of external laboratory analysis. As the Idexx Procyte Dx® currently only provides a TNCC in 10 cell/µL increments, software modification may improve agreement between the two methods. As in human medicine, automated methods may prove useful in the future for CSF analysis in addition to manual assessment, particularly in an emergency setting.
Animals, Dogs, Cell Count, Sensitivity and Specificity, Software
External DOI: https://doi.org/10.1111/jsap.13088
This record's URL: https://www.repository.cam.ac.uk/handle/1810/296767
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