Development and optimization of a hybridization technique to type the classical class I and class II B genes of the chicken MHC.
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Authors
Potts, Nicola D
Bichet, Coraline
Merat, Laurence
Guitton, Edouard
Krupa, Andrew P
Burke, Terry A
Kennedy, Lorna J
Sorci, Gabriele
Kaufman, Jim
Publication Date
2019-11-25Journal Title
Immunogenetics
ISSN
0093-7711
Publisher
Springer Nature
Volume
71
Issue
10
Pages
647-663
Language
eng
Type
Article
This Version
AM
Physical Medium
Print-Electronic
Metadata
Show full item recordCitation
Potts, N. D., Bichet, C., Merat, L., Guitton, E., Krupa, A. P., Burke, T. A., Kennedy, L. J., et al. (2019). Development and optimization of a hybridization technique to type the classical class I and class II B genes of the chicken MHC.. Immunogenetics, 71 (10), 647-663. https://doi.org/10.1007/s00251-019-01149-2
Abstract
The classical class I and class II molecules of the major histocompatibility complex (MHC) play crucial roles in immune responses to infectious pathogens and vaccines, as well as being important for autoimmunity, allergy, cancer and reproduction. These classical MHC genes are the most polymorphic known, with roughly 10,000 alleles in humans. In chickens, the MHC (also known as the BF-BL region) determines decisive resistance and susceptibility to infectious pathogens, but relatively few MHC alleles and haplotypes have been described in any detail. We describe a typing protocol for classical chicken class I (BF) and class II B (BLB) genes, based on a hybridization method called reference strand-mediated conformational analysis (RSCA). We optimize the various steps, validate the analysis using well-characterized chicken MHC haplotypes, apply the system to type some experimental lines and discover a new chicken class I allele. This work establishes a basis for typing the MHC genes of chickens worldwide, and provides an opportunity to correlate with microsatellite and with single nucleotide polymorphism (SNP) typing for approaches involving imputation.
Keywords
Animals, Chickens, Nucleic Acid Hybridization, Sequence Analysis, DNA, Genes, MHC Class I, Genes, MHC Class II, Polymorphism, Genetic, Polymorphism, Single-Stranded Conformational, Reference Standards
Sponsorship
Biotechnology and Biological Sciences Research Council (BBSRC) CASE studentship
Welcome Trust programme grant (089305/Z/09/Z)
Funder references
BBSRC (BB/H531319/1)
Wellcome Trust (089305/Z/09/Z)
WELLCOME TRUST (110106/Z/15/Z)
Identifiers
External DOI: https://doi.org/10.1007/s00251-019-01149-2
This record's URL: https://www.repository.cam.ac.uk/handle/1810/299004
Rights
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