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dc.contributor.authorCammarota, Eugeniaen
dc.contributor.authorSoriani, Chiaraen
dc.contributor.authorTaub, Raphaelleen
dc.contributor.authorMorgan, Fionaen
dc.contributor.authorSakai, Jiroen
dc.contributor.authorVeatch, Sarah Len
dc.contributor.authorBryant, Clareen
dc.contributor.authorCicuta, Pietroen
dc.date.accessioned2020-02-06T00:30:15Z
dc.date.available2020-02-06T00:30:15Z
dc.date.issued2020-02-05en
dc.identifier.issn1742-5689
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/301750
dc.description.abstractSignalling is of particular importance in immune cells, and upstream in the signalling pathway many membrane receptors are functional only as complexes, co-locating with particular lipid species. Work over the last 15 years has shown that plasma membrane lipid composition is close to a critical point of phase separation, with evidence that cells adapt their composition in ways that alter the proximity to this thermodynamical point. Macrophage cells are a key component of the innate immune system, responsive to infections, regulating the local state of inflammation. We investigate changes in the plasma membrane’s proximity to the critical point, as a response to stimulation by various pro- and anti-inflammatory agents. Pro-inflammatory (IFN- γ , Kdo-LipidA, LPS) perturbations induce an increase in the transition temperature of the GMPVs; anti-inflammatory IL4 has the opposite effect. These changes recapitulate complex plasma membrane composition changes, and are consistent with lipid criticality playing a master regulatory role: being closer to critical conditions increases membrane protein activity.
dc.description.sponsorshipResearch was funded by EUMarie Curie action ITN TransPol (EC), NIH-R01GM110052 and NSF10 MCB1552439 (SLV), Cambridge University Commonwealth, European and International Trust 11 (JS) ITN BioPol (PC), and Wellcome Trust Investigator grant 08045/Z/15/Z (CEB).
dc.format.mediumPrint-Electronicen
dc.languageengen
dc.publisherThe Royal Society
dc.rightsAll rights reserved
dc.rights.uri
dc.titleCriticality of plasma membrane lipids reflects activation state of macrophage cells.en
dc.typeArticle
prism.issueIdentifier163en
prism.publicationDate2020en
prism.publicationNameJournal of the Royal Society, Interfaceen
prism.startingPage20190803
prism.volume17en
dc.identifier.doi10.17863/CAM.48824
rioxxterms.versionofrecord10.1098/rsif.2019.0803en
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2020-02-05en
dc.contributor.orcidSakai, Jiro [0000-0002-2526-2766]
dc.contributor.orcidBryant, Clare [0000-0002-2924-0038]
dc.contributor.orcidCicuta, Pietro [0000-0002-9193-8496]
dc.identifier.eissn1742-5662
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBBSRC (BB/H021930/1)
pubs.funder-project-idWELLCOME TRUST (108045/Z/15/Z)
cam.orpheus.successTue Mar 31 10:37:21 BST 2020 - Embargo updated*
rioxxterms.freetoread.startdate2020-02-05


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