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dc.contributor.authorWalker, Denise S
dc.contributor.authorSchafer, William R
dc.date.accessioned2020-02-11T05:08:26Z
dc.date.available2020-02-11T05:08:26Z
dc.date.issued2020-01-29
dc.date.submitted2019-07-26
dc.identifier.other50597
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/301975
dc.description.abstractMechanosensation is central to a wide range of functions, including tactile and pain perception, hearing, proprioception, and control of blood pressure, but identifying the molecules underlying mechanotransduction has proved challenging. In Caenorhabditis elegans, the avoidance response to gentle body touch is mediated by six touch receptor neurons (TRNs), and is dependent on MEC-4, a DEG/ENaC channel. We show that hemichannels containing the innexin protein UNC-7 are also essential for gentle touch in the TRNs, as well as harsh touch in both the TRNs and the PVD nociceptors. UNC-7 and MEC-4 do not colocalize, suggesting that their roles in mechanosensory transduction are independent. Heterologous expression of unc-7 in touch-insensitive chemosensory neurons confers ectopic touch sensitivity, indicating a specific role for UNC-7 hemichannels in mechanosensation. The unc-7 touch defect can be rescued by the homologous mouse gene Panx1 gene, thus, innexin/pannexin proteins may play broadly conserved roles in neuronal mechanotransduction.
dc.languageen
dc.publishereLife Sciences Publications, Ltd
dc.rightsAttribution 4.0 International (CC BY 4.0)en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectResearch Article
dc.subjectNeuroscience
dc.subjectmechanosensation
dc.subjectinnexin
dc.subjectgap junctions
dc.subjectC. elegans
dc.titleDistinct roles for innexin gap junctions and hemichannels in mechanosensation
dc.typeArticle
dc.date.updated2020-02-11T05:08:25Z
prism.publicationNameeLife
prism.volume9
dc.identifier.doi10.17863/CAM.49052
dcterms.dateAccepted2020-01-28
rioxxterms.versionofrecord10.7554/elife.50597
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
datacite.contributor.supervisoreditor: Zimmer, Manuel
datacite.contributor.supervisorsenior_editor: Calabrese, Ronald L
dc.contributor.orcidWalker, Denise S [0000-0003-1534-1679]
dc.contributor.orcidSchafer, William R [0000-0002-6676-8034]
dc.identifier.eissn2050-084X
pubs.funder-project-idMedical Research Council (MC-A023-5PB91)
pubs.funder-project-idWellcome (WT103784MA)
pubs.funder-project-idNational Institutes of Health (1R21DC015652)


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)