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dc.contributor.authorLu, Meng
dc.contributor.authorKaminski, Clemens F
dc.contributor.authorSchierle, Gabriele S Kaminski
dc.date.accessioned2020-02-11T16:31:22Z
dc.date.available2020-02-11T16:31:22Z
dc.date.issued2020-02-11
dc.date.submitted2019-09-29
dc.identifier.otherpbab694e
dc.identifier.otherab694e
dc.identifier.otherpb-101118.r2
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/302010
dc.description.abstractAbstract: The aggregation of intrinsically disordered proteins is a hallmark of neurodegenerative diseases, such as Alzheimer’s, Parkinson’s and Huntington’s disease. Although we currently have a good molecular level understanding on how protein aggregation occurs in vitro, the details of its self-assembly in live cells are still mainly unknown. During the last ten years, we have witnessed the rapid development of advanced imaging techniques, especially super-resolution and fluorescence lifetime-based microscopy, in different areas of cell biology. These methods have been revolutionising our understanding of how proteins aggregate, providing unprecedented high spatial-temporal resolution which permits us to capture the kinetics of aggregate seeding and expansion, the motion and distribution of individual aggregates within the cells, and its structural change. In this article, we will review the study of in situ protein aggregation using advanced imaging techniques, with the focus on protein aggregate structure and its assembly dynamics.
dc.languageen
dc.publisherIOP Publishing
dc.rightsAttribution 3.0 Unported (CC BY 3.0)en
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/en
dc.subjectTopical Review
dc.subjectSpecial Issue on Advanced Methods for Reconstructing Molecular Dynamics from Single Cell Data
dc.subjectadvanced imaging
dc.subjectprotein aggregation
dc.subjectneurodegenerative diseases
dc.subjectsuper-resolution microscopy
dc.subjectstructural progression
dc.titleAdvanced fluorescence imaging of in situ protein aggregation
dc.typeArticle
dc.date.updated2020-02-11T16:31:21Z
prism.issueIdentifier2
prism.publicationNamePhysical Biology
prism.volume17
dc.identifier.doi10.17863/CAM.49086
dcterms.dateAccepted2020-01-08
rioxxterms.versionofrecord10.1088/1478-3975/ab694e
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/3.0/
dc.contributor.orcidLu, Meng [0000-0001-9311-2666]
dc.identifier.eissn1478-3975


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Attribution 3.0 Unported (CC BY 3.0)
Except where otherwise noted, this item's licence is described as Attribution 3.0 Unported (CC BY 3.0)