Architecture and site planning play an important role in ensuring houses receive good light and ventilation and in turn have a healthy and livable environment. To investigate the strength of association between structural factors of slum resettlement colonies buildings and the burden of tuberculosis (TB), a questionnaire-based semi-quantitative survey of 4080 households was carried out in three resettlement colonies (Lallubhai Compound, Natwar Parekh Compound and PMG colony) with questions on architectural patterns, socioeconomic details as well as occurrence of TB in any member of the household. Computational modelling for Sky View Factor, Daylight Autonomy and Natural Ventilation in the houses of all three colonies was also performed. The results show that lower floors do not have access to sufficient light and ventilation in the living area. All colonies had poor Sky View Factors, Daylight Autonomy and ventilation. Occurrence of TB was strongly associated with lower floor of the house, closed or only partially openable windows, lack of exhaust fans as well as the built environment of the houses. The study also traced back the poor conditions of light and ventilation to the relaxations in development control regulations (DCR 1991 and modifications) given to rehabilitation and slum redevelopment buildings. The study recommends better planning and architecture measures to be taken by the city government to bring improvements in housing and avert a public health crisis