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dc.contributor.authorOchi, Takashi
dc.contributor.authorQuarantotti, Valentina
dc.contributor.authorLin, Huawen
dc.contributor.authorJullien, Jerome
dc.contributor.authorRosa E Silva, Ivan
dc.contributor.authorBoselli, Francesco
dc.contributor.authorBarnabas, Deepak D
dc.contributor.authorJohnson, Christopher M
dc.contributor.authorMcLaughlin, Stephen H
dc.contributor.authorFreund, Stefan MV
dc.contributor.authorBlackford, Andrew N
dc.contributor.authorKimata, Yuu
dc.contributor.authorGoldstein, Raymond E
dc.contributor.authorJackson, Stephen P
dc.contributor.authorBlundell, Tom L
dc.contributor.authorDutcher, Susan K
dc.contributor.authorGergely, Fanni
dc.contributor.authorvan Breugel, Mark
dc.date.accessioned2020-04-15T23:30:29Z
dc.date.available2020-04-15T23:30:29Z
dc.date.issued2020-06-02
dc.identifier.issn0969-2126
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/304404
dc.description.abstractCentrioles are cylindrical assemblies whose peripheral microtubule array displays a 9-fold rotational symmetry that is established by the scaffolding protein SAS6. Centriole symmetry can be broken by centriole-associated structures, such as the striated fibers in Chlamydomonas that are important for ciliary function. The conserved protein CCDC61/VFL3 is involved in this process, but its exact role is unclear. Here, we show that CCDC61 is a paralog of SAS6. Crystal structures of CCDC61 demonstrate that it contains two homodimerization interfaces that are similar to those found in SAS6, but result in the formation of linear filaments rather than rings. Furthermore, we show that CCDC61 binds microtubules and that residues involved in CCDC61 microtubule binding are important for ciliary function in Chlamydomonas. Together, our findings suggest that CCDC61 and SAS6 functionally diverged from a common ancestor while retaining the ability to scaffold the assembly of basal body-associated structures or centrioles, respectively.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherElsevier BV
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCell Line
dc.subjectCilia
dc.subjectMicrotubules
dc.subjectHumans
dc.subjectChlamydomonas
dc.subjectAlgal Proteins
dc.subjectCell Cycle Proteins
dc.subjectMicrotubule-Associated Proteins
dc.subjectCrystallography, X-Ray
dc.subjectPhylogeny
dc.subjectProtein Conformation
dc.subjectModels, Molecular
dc.subjectProtein Multimerization
dc.subjectHEK293 Cells
dc.subjectProtein Domains
dc.titleCCDC61/VFL3 Is a Paralog of SAS6 and Promotes Ciliary Functions.
dc.typeArticle
prism.endingPage689.e11
prism.issueIdentifier6
prism.publicationDate2020
prism.publicationNameStructure
prism.startingPage674
prism.volume28
dc.identifier.doi10.17863/CAM.51486
dcterms.dateAccepted2020-04-11
rioxxterms.versionofrecord10.1016/j.str.2020.04.010
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2020-06
dc.contributor.orcidJullien, Jerome [0000-0002-7868-0021]
dc.contributor.orcidBoselli, Francesco [0000-0002-6338-6321]
dc.contributor.orcidGoldstein, Raymond [0000-0003-2645-0598]
dc.contributor.orcidJackson, Stephen [0000-0001-9317-7937]
dc.contributor.orcidBlundell, Tom [0000-0002-2708-8992]
dc.contributor.orcidGergely, Fanni [0000-0002-2441-8095]
dc.identifier.eissn1878-4186
rioxxterms.typeJournal Article/Review
pubs.funder-project-idCancer Research UK (18796)
pubs.funder-project-idWellcome Trust (206388/Z/17/Z)
pubs.funder-project-idEngineering and Physical Sciences Research Council (EP/M017982/1)
pubs.funder-project-idWellcome Trust (207510/Z/17/Z)
pubs.funder-project-idWellcome Trust (101050/Z/13/Z)
pubs.funder-project-idMedical Research Council (MR/P000479/1)
pubs.funder-project-idWellcome Trust (200814/Z/16/Z)
cam.issuedOnline2020-05-05
cam.orpheus.successWed May 13 08:52:59 BST 2020 - Embargo updated
cam.orpheus.counter3
rioxxterms.freetoread.startdate2021-04-28


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Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's licence is described as Attribution-NonCommercial-NoDerivatives 4.0 International