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dc.contributor.authorVan Dolah, Frances M.
dc.contributor.authorMorey, Jeanine S.
dc.contributor.authorMilne, Shard
dc.contributor.authorUng, André
dc.contributor.authorAnderson, Paul E.
dc.contributor.authorChinain, Mireille
dc.date.accessioned2020-04-16T01:10:34Z
dc.date.available2020-04-16T01:10:34Z
dc.date.issued2020-04-15
dc.date.submitted2020-01-29
dc.identifier.otherpone-d-20-02729
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/304410
dc.description.abstractMarine dinoflagellates produce a diversity of polyketide toxins that are accumulated in marine food webs and are responsible for a variety of seafood poisonings. Reef-associated dinoflagellates of the genus Gambierdiscus produce toxins responsible for ciguatera poisoning (CP), which causes over 50,000 cases of illness annually worldwide. The biosynthetic machinery for dinoflagellate polyketides remains poorly understood. Recent transcriptomic and genomic sequencing projects have revealed the presence of Type I modular polyketide synthases in dinoflagellates, as well as a plethora of single domain transcripts with Type I sequence homology. The current transcriptome analysis compares polyketide synthase (PKS) gene transcripts expressed in two species of Gambierdiscus from French Polynesia: a highly toxic ciguatoxin producer, G. polynesiensis, versus a non-ciguatoxic species G. pacificus, each assembled from approximately 180 million Illumina 125 nt reads using Trinity, and compares their PKS content with previously published data from other Gambierdiscus species and more distantly related dinoflagellates. Both modular and single-domain PKS transcripts were present. Single domain β-ketoacyl synthase (KS) transcripts were highly amplified in both species (98 in G. polynesiensis, 99 in G. pacificus), with smaller numbers of standalone acyl transferase (AT), ketoacyl reductase (KR), dehydratase (DH), enoyl reductase (ER), and thioesterase (TE) domains. G. polynesiensis expressed both a larger number of multidomain PKSs, and larger numbers of modules per transcript, than the non-ciguatoxic G. pacificus. The largest PKS transcript in G. polynesiensis encoded a 10,516 aa, 7 module protein, predicted to synthesize part of the polyether backbone. Transcripts and gene models representing portions of this PKS are present in other species, suggesting that its function may be performed in those species by multiple interacting proteins. This study contributes to the building consensus that dinoflagellates utilize a combination of Type I modular and single domain PKS proteins, in an as yet undefined manner, to synthesize polyketides.
dc.languageen
dc.publisherPublic Library of Science
dc.rightsAttribution 4.0 International (CC BY 4.0)en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectResearch Article
dc.subjectResearch and analysis methods
dc.subjectBiology and life sciences
dc.subjectComputer and information sciences
dc.titleTranscriptomic analysis of polyketide synthases in a highly ciguatoxic dinoflagellate, Gambierdiscus polynesiensis and low toxicity Gambierdiscus pacificus, from French Polynesia
dc.typeArticle
dc.date.updated2020-04-16T01:10:33Z
prism.issueIdentifier4
prism.publicationNamePLOS ONE
prism.volume15
dc.identifier.doi10.17863/CAM.51492
dcterms.dateAccepted2020-03-23
rioxxterms.versionofrecord10.1371/journal.pone.0231400
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
datacite.contributor.supervisoreditor: Waller, Ross Frederick
dc.contributor.orcidVan Dolah, Frances M. [0000-0003-1012-4181]
dc.identifier.eissn1932-6203


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)