Research data supporting Tunable bioactivity and mechanics of collagen-based tissue engineering constructs: A comparison of EDC-NHS, genipin and TG2 crosslinkers

Name: Research data supporting Tunable bioactivity and mechanics of collagen-based tissue engineering constructs: A comparison of EDC-NHS, genipin and TG2 crosslinkers
Published: 2020-05-13T16:44:23Z
Keywords: Crosslinking, Genipin, EDC-NHS, TG2

Nair, Malavika; Johal, Ramneek; Hamaia, Samir; Best, Serena; Cameron, Ruth

doi:10.17863/CAM.36098

Research data supporting Tunable bioactivity and mechanics of collagen-based tissue engineering constructs: A comparison of EDC-NHS, genipin and TG2 crosslinkers

Repository URI

https://www.repository.cam.ac.uk/handle/1810/305348

Repository DOI

https://doi.org/10.17863/CAM.36098

Files

Ninhydrin.py (4.14 KB)

MTSCellProliferation.py (16.19 KB)

LDHFibroblastCellAttachment.py (6.11 KB)

LDHCellDeath.py (9.49 KB)

CellSpreadingMeasurements.py (7.56 KB)

Type

Dataset

Authors

Nair, Malavika

https://orcid.org/0000-0002-5229-8991

Johal, Ramneek

Hamaia, Samir

Best, Serena

https://orcid.org/0000-0001-7866-8607

Cameron, Ruth

https://orcid.org/0000-0003-1573-4923

Description

The following data files are provided: Python files containing the raw data plotted to obtain 1) Ninhydrin.py for degree of crosslinking 2) TensileTest.py for average tensile modulus 3) LDHFibroblastCellAttachment.py for LDH cell adhesion assay 4) MTSCellProliferation.py for MTS cell proliferation assay 5) LDHCellDeath.py for LDH cytotoxicity assay 6) CellSpreadingMeasurements.py for quantitative cell values 7) Raw Mechanical Testing Data.xls for raw mechanical testing data and film measurements 8) Cell Images.zip for live-dead imaging and cell spreading images 9) PCR.py for the cycle threshold for GAPDH and the Col-I genes 10) MMPElisa.py for the raw values of the MMP-1 protein expression. 11) Swelling.py for the raw values of the water uptake of the collagen films. Please see the manuscript for further details.