Show simple item record

dc.contributor.authorSilva Martins, Walter Fabricio
dc.contributor.authorWilding, Craig Stephen
dc.contributor.authorIsaacs, Alison Taylor
dc.contributor.authorRippon, Emily Joy
dc.contributor.authorMegy, Karine
dc.contributor.authorDonnelly, Martin James
dc.date.accessioned2020-08-05T15:15:10Z
dc.date.available2020-08-05T15:15:10Z
dc.date.issued2019-08-06
dc.date.submitted2018-12-19
dc.identifier.others41598-019-47850-w
dc.identifier.other47850
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/308806
dc.description.abstractAbstract: Culex quinquefasciatus plays an important role in transmission of vector-borne diseases of public health importance, including lymphatic filariasis (LF), as well as many arboviral diseases. Currently, efforts to tackle C. quinquefasciatus vectored diseases are based on either mass drug administration (MDA) for LF, or insecticide-based interventions. Widespread and intensive insecticide usage has resulted in increased resistance in mosquito vectors, including C. quinquefasciatus. Herein, the transcriptome profile of Ugandan bendiocarb-resistant C. quinquefasciatus was explored to identify candidate genes associated with insecticide resistance. High levels of insecticide resistance were observed for five out of six insecticides tested, with the lowest mortality (0.97%) reported to permethrin, while for DDT, lambdacyhalothrin, bendiocarb and deltamethrin the mortality rate ranged from 1.63–3.29%. Resistance to bendiocarb in exposed mosquitoes was marked, with 2.04% mortality following 1 h exposure and 58.02% after 4 h. Genotyping of the G119S Ace-1 target site mutation detected a highly significant association (p < 0.0001; OR = 25) between resistance and Ace1-119S. However, synergist assays using the P450 inhibitor PBO, or the esterase inhibitor TPP resulted in markedly increased mortality (to ≈80%), suggesting a role of metabolic resistance in the resistance phenotype. Using a novel, custom 60 K whole-transcriptome microarray 16 genes significantly overexpressed in resistant mosquitoes were detected, with the P450 Cyp6z18 showing the highest differential gene expression (>8-fold increase vs unexposed controls). These results provide evidence that bendiocarb resistance in Ugandan C. quinquefasciatus is mediated by both target-site mechanisms and over-expression of detoxification enzymes.
dc.languageen
dc.publisherNature Publishing Group UK
dc.subjectArticle
dc.subject/631/208/199
dc.subject/631/208/212
dc.subject/38/61
dc.subject/38/77
dc.subject/38/90
dc.subjectarticle
dc.titleTranscriptomic analysis of insecticide resistance in the lymphatic filariasis vector Culex quinquefasciatus
dc.typeArticle
dc.date.updated2020-08-05T15:15:10Z
prism.issueIdentifier1
prism.publicationNameScientific Reports
prism.volume9
dc.identifier.doi10.17863/CAM.55894
dcterms.dateAccepted2019-07-25
rioxxterms.versionofrecord10.1038/s41598-019-47850-w
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidWilding, Craig Stephen [0000-0001-5818-2706]
dc.contributor.orcidMegy, Karine [0000-0002-2826-3879]
dc.identifier.eissn2045-2322


Files in this item

Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record