Sequential dual site-selective protein labelling enabled by lysine modification.

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Authors
Matos, Maria J
Brown, Libby
  Bernardim de Souza, Barbara
Guerreiro, Ana
Jiménez-Osés, Gonzalo
  Lopes Bernardes, Goncalo
Publication Date
2020-11-15
Journal Title
Bioorg Med Chem
ISSN
0968-0896
Publisher
Elsevier BV
Volume
28
Issue
22
Pages
115783
Language
eng
Type
Article
This Version
AM
Physical Medium
Print-Electronic
Metadata
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Citation
Matos, M. J., Brown, L., Bernardim de Souza, B., Guerreiro, A., Jiménez-Osés, G., & Lopes Bernardes, G. (2020). Sequential dual site-selective protein labelling enabled by lysine modification.. Bioorg Med Chem, 28 (22), 115783. https://doi.org/10.1016/j.bmc.2020.115783
Abstract
Methods that allow for chemical site-selective dual protein modification are scarce. Here, we provide proof-of-concept for the orthogonality and compatibility of a method for regioselective lysine modification with strategies for protein modification at cysteine and genetically encoded ketone-tagged amino acids. This sequential, orthogonal approach was applied to albumin and a therapeutic antibody to create functional dual site-selectively labelled proteins.
Keywords
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase, Lysine, Albumins, Antibodies, Molecular Structure
Sponsorship
Royal Society (URF\R\180019)
Identifiers
External DOI: https://doi.org/10.1016/j.bmc.2020.115783
This record's URL: https://www.repository.cam.ac.uk/handle/1810/310748
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International
Licence URL: https://creativecommons.org/licenses/by-nc-nd/4.0/
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Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's licence is described as Attribution-NonCommercial-NoDerivatives 4.0 International