Synthetic genome recoding: new genetic codes for new features.
Accepted version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Kuo, James
Stirling, Finn
Lau, Yu Heng https://orcid.org/0000-0001-6560-8410
Shulgina, Yekaterina https://orcid.org/0000-0001-7658-9294
Way, Jeffrey C
Abstract
Full genome recoding, or rewriting codon meaning, through chemical synthesis of entire bacterial chromosomes has become feasible in the past several years. Recoding an organism can impart new properties including non-natural amino acid incorporation, virus resistance, and biocontainment. The estimated cost of construction that includes DNA synthesis, assembly by recombination, and troubleshooting, is now comparable to costs of early stage development of drugs or other high-tech products. Here, we discuss several recently published assembly methods and provide some thoughts on the future, including how synthetic efforts might benefit from the analysis of natural recoding processes and organisms that use alternative genetic codes.
Description
Keywords
Alternative genetic code, Editing, Reassignment, Codon, DNA, Escherichia coli, Evolution, Molecular, Genes, Synthetic, Genetic Code, Genetic Engineering, Genome, Bacterial
Journal Title
Curr Genet
Conference Name
Journal ISSN
0172-8083
1432-0983
1432-0983
Volume Title
64
Publisher
Springer Science and Business Media LLC
Publisher DOI
Rights
All rights reserved
Sponsorship
Wellcome Trust (107402/Z/15/Z)