Beyond "to divide or not to divide": kinetics matter in Haematopoietic Stem Cells.
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Johnson, C., Belluschi, S., & Laurenti, E. (2020). Beyond "to divide or not to divide": kinetics matter in Haematopoietic Stem Cells.. Experimental hematology https://doi.org/10.1016/j.exphem.2020.11.003
Lifelong blood production is ensured by a population of rare and largely quiescent longlived Haematopoietic Stem Cells (HSCs). The advent of single cell technologies has recently highlighted underlying molecular and functional heterogeneity within the HSC pool. Despite heterogenous HSC behaviours, quiescence remains as the most uncontroversial unifying property of HSCs. Nonetheless, a multifaceted and complex continuum of states has recently been identified within what was previously described as just “quiescent”. Here we review such evidence and discuss how it challenges preconceived ideas on the contribution of cell cycle kinetics to HSC function. Specifically, we detail how both the frequency and kinetics of HSC division, largely determined by a network of molecular regulators linked to early G 1 , influence long-term HSC function in vivo . In addition, we present data which show that that lengthening the duration of G 1 by inhibiting CDK6 decreases lymphoid differentiation of a subset of lymphoidprimed human HSCs, thus linking cell cycle kinetics to cell fate decisions in HSCs. Finally, we reflect on how these new insights can be helpful to fully harness HSC potential in clinical applications that require ex vivo culture.
We would like to thank the Cambridge Blood and Stem Cell Biobank, specifically Joanna Baxter and the team of nurses consenting and collecting cord bloos samples; the Cambridge NIHR BRC Cell Phenotyping Hub for their flow cytometry services. E.L. is supported by a Sir Henry Dale fellowship from Wellcome/Royal Society (107630/Z/15/Z). Research in E.L.’s laboratory is supported by Wellcome, BBSRC, EHA, BIRAX, Royal Society and by core support grants by Wellcome and MRC to the Wellcome-MRC Cambridge Stem Cell Institute (203151/Z/16/Z). C.J. is supported by an MRC iCASE PhD studentship and S.B. by a CRUK Cambridge Cancer Centre PhD fellowship.
WELLCOME TRUST (107630/Z/15/Z)
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External DOI: https://doi.org/10.1016/j.exphem.2020.11.003
This record's URL: https://www.repository.cam.ac.uk/handle/1810/312747
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Licence URL: https://creativecommons.org/licenses/by-nc-nd/4.0/