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dc.contributor.authorLippert, Annaen
dc.contributor.authorDimov, Ivan Ben
dc.contributor.authorWinkel, Alexander Ken
dc.contributor.authorHumphrey, Janeen
dc.contributor.authorMcColl, Jamesen
dc.contributor.authorChen, Kevin Yen
dc.contributor.authorSantos, Ana Men
dc.contributor.authorJenkins, Edwarden
dc.contributor.authorFranze, Kristianen
dc.contributor.authorDavis, Simon Jen
dc.contributor.authorKlenerman, Daviden
dc.date.accessioned2020-11-24T15:27:29Z
dc.date.available2020-11-24T15:27:29Z
dc.date.issued2021-01en
dc.identifier.issn0006-3495
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/313254
dc.description.abstractMuch of what we know about the early stages of T-cell activation has been obtained from studies of T cells interacting with glass-supported lipid bilayers that favor imaging but are orders-of-magnitude stiffer than typical cells. We developed a method for attaching lipid bilayers to polydimethylsiloxane polymer supports, producing ‘soft bilayers’ with physiological levels of mechanical resistance (Young’s modulus of 4 kPa). Comparisons of T-cell behavior on soft and glass-supported bilayers revealed that, whereas late stages of T-cell activation are thought to be substrate-stiffness dependent, early calcium signaling was unaffected by substrate rigidity, implying that early steps in T-cell receptor triggering are not mechanosensitive. The exclusion of large receptor-type phosphatases was observed on the soft bilayers, however, even though it is yet to be demonstrated at authentic cell-cell contacts. This work sets the stage for an imaging-based exploration of receptor signaling under conditions closely mimicking physiological cell-cell contact.
dc.description.sponsorshipRoyal Society
dc.format.mediumPrint-Electronicen
dc.languageengen
dc.publisherBiophysical Society
dc.rightsAttribution 4.0 International (CC BY)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectT-Lymphocytesen
dc.subjectDimethylpolysiloxanesen
dc.subjectLipid Bilayersen
dc.subjectCell Communicationen
dc.subjectElastic Modulusen
dc.titleSoft Polydimethylsiloxane-Supported Lipid Bilayers for Studying T Cell Interactions.en
dc.typeArticle
prism.endingPage45
prism.issueIdentifier1en
prism.publicationDate2021en
prism.publicationNameBiophysical journalen
prism.startingPage35
prism.volume120en
dc.identifier.doi10.17863/CAM.60359
dcterms.dateAccepted2020-11-17en
rioxxterms.versionofrecord10.1016/j.bpj.2020.11.021en
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2021-01en
dc.contributor.orcidLippert, Anna [0000-0002-0771-8491]
dc.contributor.orcidHumphrey, Jane [0000-0002-6825-1426]
dc.contributor.orcidFranze, Kristian [0000-0002-8425-7297]
dc.contributor.orcidKlenerman, David [0000-0001-7116-6954]
dc.identifier.eissn1542-0086
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idEPSRC (EP/L015978/1)
pubs.funder-project-idEuropean Commission Horizon 2020 (H2020) ERC (772426)
pubs.funder-project-idWellcome Trust (via University of Oxford) (207547/Z/17/Z)
cam.orpheus.successMon Nov 30 07:30:28 GMT 2020 - Embargo updated*
rioxxterms.freetoread.startdate2100-01-01


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Attribution 4.0 International (CC BY)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY)