Revision in the first steps of the biosynthesis of the red antibiotic prodigiosin: use of a synthetic thioester to validate a new intermediate.
Bhalara, Hiral D
Monson, Rita E
Salmond, George PC
RSC chemical biology
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Couturier, M., Bhalara, H. D., Monson, R. E., Salmond, G. P., & Leeper, F. (2021). Revision in the first steps of the biosynthesis of the red antibiotic prodigiosin: use of a synthetic thioester to validate a new intermediate.. RSC chemical biology, 2 (2), 551-555. https://doi.org/10.1039/d0cb00173b
A biosynthetic pathway for the red-antibiotic, prodigiosin, was proposed over a decade ago but not all the suggested intermediates could be detected experimentally. Here we show that a thioester that was not originally included in the pathway is an intermediate. In addition, the enzyme PigE was originally described as a transaminase but we present evidence that it also catalyses the reduction of the thioester intermediate to its aldehyde substrate.
BBSRC, UK, awards BB/N008081/1 and BB/K001833/1 Frances and Augustus Newman foundation Cambridge Commonwealth Trust Emmanuel College, Cambridge
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External DOI: https://doi.org/10.1039/d0cb00173b
This record's URL: https://www.repository.cam.ac.uk/handle/1810/315814
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/