Additional Materials of the Thesis “Investigating the cell surface code for compartment boundary formation in Drosophila embryos.” Thomas Edward Sharrock
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Movie01: Imaging nascent mRNA directed by EnVT15159. Movie showing transcription from EnVT15159-pEve in the Drosophila embryonic ectoderm during germ-band extension. Maximum Intensity Projection (31x1μm stacks) of the MCP-GFP channel. Time resolution of 30s per frame. Anterior to Left, Ventral Down.
Movie02: Gap43-mCherry reveals cell membranes. Movie showing Gap43-mCherry protein localising to cell membranes in the Drosophila embryonic ectoderm during germ-band extension. Maximum Intensity Projection (31x1μm stacks) of the Gap43-mCherry channel. Time resolution of 30s per frame. Anterior to Left, Ventral Down.
Movie03: Blanketed projection of Gap43-mCherry. Movie showing projection of Gap43-mCherry signal following blanketing in oTracks. Maximum Intensity Projection (2x1μm stacks) of the most apical Gap43-mCherry signal. Time resolution of 30s per frame. Anterior to Left, Ventral Down.
Movie04: Segmentation and Cell Tracking. Movie showing cell segmentation and cell tracking undertaken in otracks using the Gap43-mCherry signal. Time resolution of 30s per frame. Anterior to Left, Ventral Down.
Movie05: Dot:Cell assignment. Movie showing EnVT15159 directed transcriptional dots assigned to cells in oTracks. Fire LUT of cell outlines indicates probability a cell has a transcriptional dot located within it (Red=Low Probability, Blue=High Probability). Underlaid is maximum intensity projection of MCP-GFP channel as described in Movie01. Time resolution of 30s per frame. Anterior to Left, Ventral Down.
Movie06: Locating and quantifying straightness at PSBs. Movie showing PSBs located using the EnVT15159 directed transcriptional dots. Cell positions are represented by squares at each cell’s centroid. Squares are coloured dependent upon parasegment identity. At the boundary between parasegments is where PSB interfaces are located. Time resolution of 30s per frame. Anterior to Left, Ventral Down.
Movie07: Marching boundary method. Movie showing the marching boundary process ran on oTracks. Boundary paths explore all possible paths across double parasegment repeats. The straightest, non-overlapping paths are chosen, and their index of straightness measures are plotted across a double parasegment coordinate system. Anterior Up, Posterior Down.
