This thesis addresses two aspects of visual processing in two different invertebrate organisms. The fruit fly, Drosophila melanogaster, has emerged as a key model for invertebrate vision research. Despite extensive characterisation of motion vision, very little is known about how flies process colour information, or how the spectral content of light affects other visual modalities. With the aim to accurately dissect the different components of the Drosophila visual system responsible for processing colour, I have developed a versatile visual stimulation setup to probe for the combinations of spatial, temporal and spectral visual response properties. Using flies that express neural activity indicators, I can track visual responses to a colour stimulus (i.e. narrow bands of light across the spectrum) via a two-photon imaging system. The visual stimulus is projected on a specialised screen material that scatters wavelengths of light across the spectrum equally at all locations of the screen, thus enabling presentation of spatially structured stimuli. Using this setup, I have characterised spectral responses, intensity-response relationships, and receptive fields of neurons in the early visual system of a variety of genetically modified strains of Drosophila. Specifically, I compared visual responses in the medulla of flies expressing either a subset or all photoreceptor opsins, with differing levels of screening pigment present in the eye. I found layer-specific shifts of spectral response properties correlating with projection regions of photoreceptor terminals. I also 3 found that a reduction in screening pigment shifts the general spectral response in the neuropil towards the longer wavelengths of light. I have also mapped receptive fields across the different layers of the medulla for the peak spectral response wavelength. My results suggest that receptive field dimensions match the expected size predicted by the conservation of a columnar organisation in the medulla, with little variation from layer to layer. In a subset of these cells, we see an elongated receptive field suggestive of static orientation selectivity with an apparent split in the preferred axis of orientation of these receptive fields, with a near-orthogonal angle between the summed vectors of the split populations. The camera type eyes of vertebrates and cephalopods exhibit remarkable convergence, but it is currently unknown if the mechanisms for visual information processing in these brains, which exhibit wildly disparate architecture, is also shared. I chose to investigate the visual processing mechanism known as stereopsis in the cuttlefish Sepia officinalis. Stereoscopic vision is used to assess depth information by comparing the disparity between left and right visual fields. This strategy is commonplace in vertebrates having evolved multiple times independently but has only been demonstrated in one invertebrate: the praying mantis. Cuttlefish require precise distance estimation during their predatory hunt when they extend two tentacles in a ballistic strike to catch their target. Using a 3D perception paradigm whereby the cuttlefish were fitted with anaglyph glasses, I show that these animals use stereopsis to resolve distance to their prey. Although this is not an exclusive depth perception mechanism for hunting, it does shorten the time and distance covered prior to striking at a target. Furthermore, stereopsis in cuttlefish works differently to vertebrates, as cuttlefish can extract stereopsis cues from anti-correlated stimuli.