Show simple item record

dc.contributor.authorGardner, Michael J
dc.contributor.authorBaker, Andrew J
dc.contributor.authorAssie, Jean-Maurice
dc.contributor.authorPoethig, R Scott
dc.contributor.authorHaseloff, Jim
dc.contributor.authorWebb, Alex
dc.date.accessioned2021-10-07T23:30:34Z
dc.date.available2021-10-07T23:30:34Z
dc.date.issued2009
dc.identifier.issn0022-0957
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/329118
dc.description.abstractTo facilitate the monitoring of guard cells during development and isolation, a population of 704 GAL4 GFP enhancer trap lines was screened and four single insert lines with guard cell GFP expression and one with developmentally-regulated guard cell GFP expression were identified. The location of the T-DNA inserts, the expression of the flanking genes, and the promoter activity of the genomic DNA upstream of the T-DNA were characterized. The results indicated that the GFP expression pattern in at least one of the lines was due to elements in the intergenic DNA immediately upstream of the T-DNA, rather than due to the activity of the promoters of genes flanking the insert, and provide evidence for the involvement of Dof elements in regulating guard cell gene expression. It is shown further that the GAL4 GFP lines can be used to track the contribution of guard cell material in vitro, and this method was used to assess the purity of guard cell samples obtained using two methods of guard cell isolation.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherOxford University Press (OUP)
dc.rightsAttribution-NonCommercial 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectArabidopsis
dc.subjectTrans-Activators
dc.subjectGreen Fluorescent Proteins
dc.subjectMutagenesis, Insertional
dc.subjectGene Expression Regulation, Developmental
dc.subjectGene Expression Regulation, Plant
dc.subjectPlant Stomata
dc.subjectEnhancer Elements, Genetic
dc.titleGAL4 GFP enhancer trap lines for analysis of stomatal guard cell development and gene expression.
dc.typeArticle
prism.endingPage226
prism.issueIdentifier1
prism.publicationDate2009
prism.publicationNameJ Exp Bot
prism.startingPage213
prism.volume60
dc.identifier.doi10.17863/CAM.76564
rioxxterms.versionofrecord10.1093/jxb/ern292
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2009-01
dc.contributor.orcidHaseloff, Jim [0000-0003-4793-8058]
dc.contributor.orcidWebb, Alex [0000-0003-0261-4375]
dc.identifier.eissn1460-2431
rioxxterms.typeJournal Article/Review
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/D017904/1)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/F011458/1)
cam.issuedOnline2008-11-25


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial 4.0 International
Except where otherwise noted, this item's licence is described as Attribution-NonCommercial 4.0 International