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dc.contributor.authorFairman, Connor W.
dc.contributor.authorLever, Andrew M. L.
dc.contributor.authorKenyon, Julia C.
dc.date.accessioned2021-10-24T00:32:10Z
dc.date.available2021-10-24T00:32:10Z
dc.date.issued2021-10-22
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/329818
dc.description.abstractOur understanding of RNA structure has lagged behind that of proteins and most other biological polymers, largely because of its ability to adopt multiple, and often very different, functional conformations within a single molecule. Flexibility and multifunctionality appear to be its hallmarks. Conventional biochemical and biophysical techniques all have limitations in solving RNA structure and to address this in recent years we have seen the emergence of a wide diversity of techniques applied to RNA structural analysis and an accompanying appreciation of its ubiquity and versatility. Viral RNA is a particularly productive area to study in that this economy of function within a single molecule admirably suits the minimalist lifestyle of viruses. Here, we review the major techniques that are being used to elucidate RNA conformational flexibility and exemplify how the structure and function are, as in all biology, tightly linked.
dc.languageen
dc.publisherMDPI
dc.subjectRNA structure
dc.subjectRNA flexibility
dc.subjectRNA viruses
dc.subjectSHAPE
dc.subjectproximity ligation
dc.subjectNMR
dc.subjectSAXS
dc.subjectsmFRET
dc.titleEvaluating RNA Structural Flexibility: Viruses Lead the Way
dc.typeArticle
dc.date.updated2021-10-24T00:32:09Z
prism.issueIdentifier11
prism.publicationNameViruses
prism.volume13
dc.identifier.doi10.17863/CAM.77263
dcterms.dateAccepted2021-10-18
rioxxterms.versionofrecord10.3390/v13112130
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidKenyon, Julia C. [0000-0001-6055-7052]
dc.identifier.eissn1999-4915


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