Insulin Tolerance Test under Anaesthesia to Measure Tissue-specific Insulin-stimulated Glucose Disposal.
Fazakerley, Daniel J
Fritzen, Andreas M
Nelson, Marin E
Thorius, Ida H
Cooke, Kristen C
Humphrey, Sean J
Cooney, Gregory J
James, David E
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Fazakerley, D. J., Fritzen, A. M., Nelson, M. E., Thorius, I. H., Cooke, K. C., Humphrey, S. J., Cooney, G. J., & et al. (2019). Insulin Tolerance Test under Anaesthesia to Measure Tissue-specific Insulin-stimulated Glucose Disposal.. Bio Protoc, 9 (2), e3146. https://doi.org/10.21769/BioProtoc.3146
Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic clamps permit assessment of insulin responses in specific tissues and allow the study of the progression and causes of insulin resistance. Here we detail a protocol for assessing insulin action in adipose and muscle tissues in anesthetized mice administered with insulin intravenously.
External DOI: https://doi.org/10.21769/BioProtoc.3146
This record's URL: https://www.repository.cam.ac.uk/handle/1810/330681
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/