Insulin Tolerance Test under Anaesthesia to Measure Tissue-specific Insulin-stimulated Glucose Disposal.
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Authors
Fazakerley, Daniel J
Fritzen, Andreas M
Nelson, Marin E
Thorius, Ida H
Cooke, Kristen C
Humphrey, Sean J
Cooney, Gregory J
James, David E
Publication Date
2019-01-20Journal Title
Bio Protoc
ISSN
2331-8325
Publisher
Bio-Protocol, LLC
Volume
9
Issue
2
Pages
e3146
Language
eng
Type
Article
This Version
VoR
Physical Medium
Electronic-eCollection
Metadata
Show full item recordCitation
Fazakerley, D. J., Fritzen, A. M., Nelson, M. E., Thorius, I. H., Cooke, K. C., Humphrey, S. J., Cooney, G. J., & et al. (2019). Insulin Tolerance Test under Anaesthesia to Measure Tissue-specific Insulin-stimulated Glucose Disposal.. Bio Protoc, 9 (2), e3146. https://doi.org/10.21769/BioProtoc.3146
Abstract
Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic clamps permit assessment of insulin responses in specific tissues and allow the study of the progression and causes of insulin resistance. Here we detail a protocol for assessing insulin action in adipose and muscle tissues in anesthetized mice administered with insulin intravenously.
Keywords
Adipose tissue, Glucose transport, Glucose uptake, Insulin, Insulin tolerance test, Muscle
Identifiers
External DOI: https://doi.org/10.21769/BioProtoc.3146
This record's URL: https://www.repository.cam.ac.uk/handle/1810/330681
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