Dataset for colocalization analysis
Description
Zip folder containing set of images (.tif files) analysed in the paper: “The SARS-CoV-2 nucleocapsid protein associates with the replication organelles before viral assembly at the Golgi/ERGIC and lysosome-mediated egress”, doi: 10.1101/2021.06.15.448497.
Fixed Vero cells were imaged with confocal microscope (pixel size is 70.6 nm). Zip folder contains images stained for different viral proteins: Spike (S), Envelope (E), Membrane (M), Nucleocapsid (N), lysosomes, ERGIC complex and Golgi apparatus. Images are already automatically cropped around each single cell (see associated article methods for automatic cropping details). Description of colors in each subset is present in txt files which are included in all subsets. These images were analysed for Manders and Spearman coefficients with ColocAnalyzer v.4 https://doi.org/10.17863/CAM.77726
For more details see associated manuscript.
Format
Dataset was analysed using ColocAnalyzer v.4: https://doi.org/10.17863/CAM.77726
Keywords
SARS-COV-2, Envelope protein, Spike protein, Nucleocapsid protein, Membrane protein, Golgi, ERGIC
Relationships
Publication Reference: https://doi.org/10.1126/sciadv.abl4895https://www.repository.cam.ac.uk/handle/1810/332628
Identifiers
This record's DOI: https://doi.org/10.17863/CAM.78118
Rights
Attribution 4.0 International (CC BY 4.0)
Licence URL: https://creativecommons.org/licenses/by/4.0/
Statistics
Total file downloads (since January 2020). For more information on metrics see the
IRUS guide.
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)