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dc.contributor.authorKnapper, Joe
dc.contributor.authorCollins, Joel T
dc.contributor.authorStirling, Julian
dc.contributor.authorMcDermott, Samuel
dc.contributor.authorWadsworth, William
dc.contributor.authorBowman, Richard W
dc.date.accessioned2021-11-22T14:37:24Z
dc.date.available2021-11-22T14:37:24Z
dc.date.issued2022-01
dc.date.submitted2021-07-23
dc.identifier.issn0022-2720
dc.identifier.otherjmi13064
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/330810
dc.description.abstractThe OpenFlexure Microscope is a 3D-printed, low-cost microscope capable of automated image acquisition through the use of a motorised translation stage and a Raspberry Pi imaging system. This automation has applications in research and healthcare, including in supporting the diagnosis of malaria in low-resource settings. The plasmodium parasites that cause malaria require high magnification imaging, which has a shallow depth of field, necessitating the development of an accurate and precise autofocus procedure. We present methods of identifying the focal plane of the microscope, and procedures for reliably acquiring a stack of focused images on a system affected by backlash and drift. We also present and assess a method to verify the success of autofocus during the scan. The speed, reliability and precision of each method are evaluated, and the limitations discussed in terms of the end users' requirements.
dc.languageen
dc.publisherWiley
dc.subjectORIGINAL ARTICLE
dc.subjectORIGINAL ARTICLES
dc.subjectautofocus
dc.subjectfocus
dc.subjectlight microscopy
dc.subjectopen hardware
dc.subjectopen source
dc.titleFast, high-precision autofocus on a motorised microscope: Automating blood sample imaging on the OpenFlexure Microscope.
dc.typeArticle
dc.date.updated2021-11-22T14:37:24Z
prism.publicationNameJ Microsc
dc.identifier.doi10.17863/CAM.78253
dcterms.dateAccepted2021-09-28
rioxxterms.versionofrecord10.1111/jmi.13064
rioxxterms.versionAO
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidKnapper, Joe [0000-0002-5519-1700]
dc.contributor.orcidCollins, Joel T [0000-0002-9382-7511]
dc.contributor.orcidStirling, Julian [0000-0002-8270-9237]
dc.contributor.orcidMcDermott, Samuel [0000-0003-2736-5467]
dc.contributor.orcidWadsworth, William [0000-0003-4733-7594]
dc.contributor.orcidBowman, Richard W [0000-0002-1531-8199]
dc.identifier.eissn1365-2818
pubs.funder-project-idEngineering and Physical Sciences Research Council (EP/R011443/1)
pubs.funder-project-idEngineering and Physical Sciences Research Council (EP/R013969/1)
cam.issuedOnline2021-11-14


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