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dc.contributor.authorMinshall, Nicola
dc.contributor.authorChernukhin, Igor
dc.contributor.authorCarroll, Jason
dc.contributor.authorGit, Anna
dc.date.accessioned2021-12-15T11:10:26Z
dc.date.available2021-12-15T11:10:26Z
dc.date.issued2021-11-29
dc.date.submitted2021-10-10
dc.identifier.issn0736-6205
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/331460
dc.description.abstractDespite their abundance, mid-sized RNAs (30-300 nt) have not been extensively studied by high-throughput sequencing, mostly due to selective loss in library preparation. The authors propose simple and inexpensive modifications to the Illumina TruSeq protocol (ncRNAseq), allowing the capture and sequencing of mid-sized non-coding RNAs without detriment to the coverage of coding mRNAs. This protocol is coupled with a two-step alignment: a pre-alignment to a curated non-coding genome, passing only the non-mapping reads to a standard genomic alignment. ncRNAseq correctly assigns the highest read-numbers to established abundant non-coding RNAs and correctly identifies cytosolic and nuclear enrichment of known non-coding RNAs in two cell lines.
dc.languageen
dc.publisherFuture Science Ltd
dc.subjectReports
dc.subject5′ coverage
dc.subjecthigh-throughput sequencing
dc.subjectnext-generation sequencing
dc.subjectnon-coding RNA
dc.subjectsize-selection
dc.titlencRNAseq: simple modifications to RNA-seq library preparation allow recovery and analysis of mid-sized non-coding RNAs.
dc.typeArticle
dc.date.updated2021-12-15T11:10:25Z
prism.issueIdentifier0
prism.publicationNameBiotechniques
prism.volume0
dc.identifier.doi10.17863/CAM.78914
dcterms.dateAccepted2021-11-09
rioxxterms.versionofrecord10.2144/btn-2021-0035
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.contributor.orcidMinshall, Nicola [0000-0002-8350-4903]
dc.contributor.orcidChernukhin, Igor [0000-0002-4411-1218]
dc.contributor.orcidCarroll, Jason [0000-0003-3643-0080]
dc.contributor.orcidGit, Anna [0000-0003-0973-9138]
dc.identifier.eissn1940-9818
pubs.funder-project-idBreast Cancer Now (2016NovPR816)
cam.issuedOnline2021-11-29
datacite.issupplementedby.urlhttps://doi.org/10.17863/CAM.66304


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