Show simple item record

dc.contributor.authorLanham, Stuart A
dc.contributor.authorBlache, Dominique
dc.contributor.authorOreffo, Richard OC
dc.contributor.authorFowden, Abigail L
dc.contributor.authorForhead, Alison J
dc.date.accessioned2022-01-06T12:56:42Z
dc.date.available2022-01-06T12:56:42Z
dc.date.issued2021-11-24
dc.identifier.issn0022-0795
dc.identifier.otherPMC8630774
dc.identifier.other34708692
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332225
dc.description.abstractHormones have an important role in the regulation of fetal growth and development, especially in response to nutrient availability in utero. Using micro-CT and an electromagnetic three-point bend test, this study examined the effect of pancreas removal at 0.8 fraction of gestation on the developing bone structure and mechanical strength in fetal sheep. When fetuses were studied at 10 and 25 days after surgery, pancreatectomy caused hypoinsulinaemia, hyperglycaemia and growth retardation which was associated with low plasma concentrations of leptin and a marker of osteoclast activity and collagen degradation. In pancreatectomized fetuses compared to control fetuses, limb lengths were shorter, and trabecular (Tb) bone in the metatarsi showed greater bone volume fraction, Tb thickness, degree of anisotropy and porosity, and lower fractional bone surface area and Tb spacing. Mechanical strength testing showed that pancreas deficiency was associated with increased stiffness and a greater maximal weight load at fracture in a subset of fetuses studied near term. Overall, pancreas deficiency in utero slowed the growth of the fetal skeleton and adapted the developing bone to generate a more compact and connected structure. Maintenance of bone strength in growth-retarded limbs is especially important in a precocial species in preparation for skeletal loading and locomotion at birth.
dc.languageeng
dc.publisherBioscientifica
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 1479-6805
dc.sourcenlmid: 0375363
dc.subjectPancreas
dc.subjectInsulin
dc.subjectSkeleton
dc.subjectGlucose
dc.subjectLeptin
dc.subjectFetus
dc.subjectBone Structure
dc.subjectMetatarsal
dc.subjectMicro-computed Tomography
dc.titlePancreas deficiency modifies bone development in the ovine fetus near term.
dc.typeArticle
dc.date.updated2022-01-06T12:56:42Z
prism.endingPage80
prism.issueIdentifier1
prism.publicationNameJ Endocrinol
prism.startingPage71
prism.volume252
dc.identifier.doi10.17863/CAM.79671
dcterms.dateAccepted2021-10-27
rioxxterms.versionofrecord10.1530/JOE-21-0189
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidFowden, Abigail [0000-0002-3384-4467]
dc.contributor.orcidForhead, Alison [0000-0003-2125-4811]
dc.identifier.eissn1479-6805
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/H01697X/1)
cam.issuedOnline2021-11-24


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International