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dc.contributor.authorKeller, Christian
dc.contributor.authorRakotozandrindrainy, Raphaël
dc.contributor.authorvon Kalckreuth, Vera
dc.contributor.authorHeriniaina, Jean Noël
dc.contributor.authorSchwarz, Norbert Georg
dc.contributor.authorPak, Gi Deok
dc.contributor.authorIm, Justin
dc.contributor.authorEspinoza, Ligia Maria Cruz
dc.contributor.authorHagen, Ralf Matthias
dc.contributor.authorFrickmann, Hagen
dc.contributor.authorRakotondrainiarivelo, Jean Philibert
dc.contributor.authorRazafindrabe, Tsiry
dc.contributor.authorDekker, Denise
dc.contributor.authorMay, Jürgen
dc.contributor.authorPoppert, Sven
dc.contributor.authorMarks, Florian
dc.date.accessioned2022-01-07T16:54:14Z
dc.date.available2022-01-07T16:54:14Z
dc.date.issued2021-11-14
dc.identifier.citationPathogens (Basel, Switzerland), volume 10, issue 11
dc.identifier.issn2076-0817
dc.identifier.otherPMC8621948
dc.identifier.other34832637
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332437
dc.description.abstractRickettsiae may cause febrile infections in humans in tropical and subtropical regions. From Madagascar, no molecular data on the role of rickettsioses in febrile patients are available. Blood samples from patients presenting with fever in the area of the capital Antananarivo were screened for the presence of rickettsial DNA. EDTA (ethylenediaminetetraacetic acid) blood from 1020 patients presenting with pyrexia > 38.5 °C was analyzed by gltA-specific qPCR. Positive samples were confirmed by ompB-specific qPCR. From confirmed samples, the gltA amplicons were sequenced and subjected to phylogenetic analysis. From five gltA-reactive samples, two were confirmed by ompB-specific qPCR. The gltA sequence in the sample taken from a 38-year-old female showed 100% homology with R. typhi. The other sample taken from a 1.5-year-old infant was 100% homologous to R. felis. Tick-borne rickettsiae were not identified. The overall rate of febrile patients with molecular evidence for a rickettsial infection from the Madagascan study site was 0.2% (2/1020 patients). Flea-borne rickettsiosis is a rare but neglected cause of infection in Madagascar. Accurate diagnosis may prompt adequate antimicrobial treatment.
dc.languageeng
dc.publisherMDPI AG
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 2076-0817
dc.sourcenlmid: 101596317
dc.subjectRickettsia Typhi
dc.subjectMadagascar
dc.subjectRickettsia Felis
dc.subjectMurine Typhus
dc.subjectFlea-borne Rickettsiosis
dc.titleMolecular Evidence for Flea-Borne Rickettsiosis in Febrile Patients from Madagascar.
dc.typeArticle
dc.date.updated2022-01-07T16:54:13Z
prism.publicationNamePathogens
dc.identifier.doi10.17863/CAM.79883
dcterms.dateAccepted2021-11-09
rioxxterms.versionofrecord10.3390/pathogens10111482
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidKeller, Christian [0000-0002-0075-7199]
dc.contributor.orcidHagen, Ralf Matthias [0000-0003-4875-1519]
dc.contributor.orcidFrickmann, Hagen [0000-0002-8967-9528]
dc.contributor.orcidDekker, Denise [0000-0001-5432-3217]
dc.contributor.orcidPoppert, Sven [0000-0003-4873-249X]
dc.contributor.orcidMarks, Florian [0000-0002-6043-7170]
dc.identifier.eissn2076-0817
pubs.funder-project-idBill & Melinda Gates Foundation (OPPGH5231)
pubs.funder-project-idBill &amp (OPPGH5231)
cam.issuedOnline2021-11-14


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International