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dc.contributor.authorSpitz, Olivia
dc.contributor.authorErenburg, Isabelle N
dc.contributor.authorKanonenberg, Kerstin
dc.contributor.authorPeherstorfer, Sandra
dc.contributor.authorLenders, Michael HH
dc.contributor.authorReiners, Jens
dc.contributor.authorMa, Miao
dc.contributor.authorLuisi, Ben
dc.contributor.authorSmits, Sander HJ
dc.contributor.authorSchmitt, Lutz
dc.date.accessioned2022-01-15T00:30:40Z
dc.date.available2022-01-15T00:30:40Z
dc.date.issued2021
dc.identifier.issn1664-042X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332731
dc.description.abstractThe toxin hemolysin A was first identified in uropathogenic E. coli strains and shown to be secreted in a one-step mechanism by a dedicated secretion machinery. This machinery, which belongs to the Type I secretion system family of the Gram-negative bacteria, is composed of the outer membrane protein TolC, the membrane fusion protein HlyD and the ABC transporter HlyB. The N-terminal domain of HlyA represents the toxin which is followed by a RTX (Repeats in Toxins) domain harboring nonapeptide repeat sequences and the secretion signal at the extreme C-terminus. This secretion signal, which is necessary and sufficient for secretion, does not appear to require a defined sequence, and the nature of the encoded signal remains unknown. Here, we have combined structure prediction based on the AlphaFold algorithm together with functional and in silico data to examine the role of secondary structure in secretion. Based on the presented data, a C-terminal, amphipathic helix is proposed between residues 975 and 987 that plays an essential role in the early steps of the secretion process.
dc.description.sponsorshipERC
dc.publisherFrontiers Media
dc.rightsAll Rights Reserved
dc.rights.urihttp://www.rioxx.net/licenses/all-rights-reserved
dc.titleIdentity Determinants of the Translocation Signal for a Type 1 Secretion System.
dc.typeArticle
dc.publisher.departmentDepartment of Biochemistry
dc.date.updated2022-01-13T13:36:56Z
prism.publicationNameFront Physiol
dc.identifier.doi10.17863/CAM.80172
dcterms.dateAccepted2021-12-31
rioxxterms.versionofrecord10.3389/fphys.2021.804646
rioxxterms.versionAM
dc.contributor.orcidLuisi, Ben [0000-0003-1144-9877]
dc.identifier.eissn1664-042X
rioxxterms.typeJournal Article/Review
pubs.funder-project-idEuropean Research Council (742210)
cam.orpheus.successWed Mar 23 10:26:23 GMT 2022 - Embargo updated*
cam.orpheus.counter2
cam.depositDate2022-01-13
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement
rioxxterms.freetoread.startdate2021-12-31


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