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dc.contributor.authorBieluszewski, Tomasz
dc.contributor.authorSura, Weronika
dc.contributor.authorDziegielewski, Wojciech
dc.contributor.authorBieluszewska, Anna
dc.contributor.authorLachance, Catherine
dc.contributor.authorKabza, Michał
dc.contributor.authorSzymanska-Lejman, Maja
dc.contributor.authorAbram, Mateusz
dc.contributor.authorWlodzimierz, Piotr
dc.contributor.authorDe Winne, Nancy
dc.contributor.authorDe Jaeger, Geert
dc.contributor.authorSadowski, Jan
dc.contributor.authorCôté, Jacques
dc.contributor.authorZiolkowski, Piotr A
dc.date.accessioned2022-03-11T00:30:58Z
dc.date.available2022-03-11T00:30:58Z
dc.date.issued2022-01-12
dc.identifier.issn2041-1723
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/334874
dc.description.abstractNucleosomal acetyltransferase of H4 (NuA4) is an essential transcriptional coactivator in eukaryotes, but remains poorly characterized in plants. Here, we describe Arabidopsis homologs of the NuA4 scaffold proteins Enhancer of Polycomb-Like 1 (AtEPL1) and Esa1-Associated Factor 1 (AtEAF1). Loss of AtEAF1 results in inhibition of growth and chloroplast development. These effects are stronger in the Atepl1 mutant and are further enhanced by loss of Golden2-Like (GLK) transcription factors, suggesting that NuA4 activates nuclear plastid genes alongside GLK. We demonstrate that AtEPL1 is necessary for nucleosomal acetylation of histones H4 and H2A.Z by NuA4 in vitro. These chromatin marks are diminished genome-wide in Atepl1, while another active chromatin mark, H3K9 acetylation (H3K9ac), is locally enhanced. Expression of many chloroplast-related genes depends on NuA4, as they are downregulated with loss of H4ac and H2A.Zac. Finally, we demonstrate that NuA4 promotes H2A.Z deposition and by doing so prevents spurious activation of stress response genes.
dc.format.mediumElectronic
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAcetyltransferases
dc.subjectArabidopsis
dc.subjectArabidopsis Proteins
dc.subjectAutotrophic Processes
dc.subjectCell Nucleus
dc.subjectChloroplasts
dc.subjectChromatin
dc.subjectEphrin-A1
dc.subjectGene Expression Regulation, Plant
dc.subjectHistones
dc.subjectNuclear Pore Complex Proteins
dc.subjectNucleosomes
dc.subjectStress, Physiological
dc.subjectTranscription Factors
dc.titleNuA4 and H2A.Z control environmental responses and autotrophic growth in Arabidopsis.
dc.typeArticle
dc.publisher.departmentDepartment of Plant Sciences
dc.date.updated2022-03-10T09:14:34Z
prism.issueIdentifier1
prism.numberARTN 277
prism.publicationDate2022
prism.publicationNameNat Commun
prism.startingPage277
prism.volume13
dc.identifier.doi10.17863/CAM.82311
dcterms.dateAccepted2021-12-21
rioxxterms.versionofrecord10.1038/s41467-021-27882-5
rioxxterms.versionVoR
dc.contributor.orcidBieluszewski, Tomasz [0000-0001-6154-5213]
dc.contributor.orcidSura, Weronika [0000-0003-0304-3615]
dc.contributor.orcidDziegielewski, Wojciech [0000-0002-5517-4839]
dc.contributor.orcidBieluszewska, Anna [0000-0003-3873-0802]
dc.contributor.orcidSzymanska-Lejman, Maja [0000-0003-4813-3278]
dc.contributor.orcidAbram, Mateusz [0000-0003-0492-4625]
dc.contributor.orcidWlodzimierz, Piotr [0000-0003-1040-7878]
dc.contributor.orcidCôté, Jacques [0000-0001-6751-555X]
dc.contributor.orcidZiolkowski, Piotr A [0000-0001-7673-6565]
dc.identifier.eissn2041-1723
rioxxterms.typeJournal Article/Review
cam.issuedOnline2022-01-12
cam.depositDate2022-03-10
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International