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dc.contributor.authorGoubert, Clement
dc.contributor.authorCraig, Rory J
dc.contributor.authorBilat, Agustin F
dc.contributor.authorPeona, Valentina
dc.contributor.authorVogan, Aaron A
dc.contributor.authorProtasio, Anna V
dc.date.accessioned2022-03-31T16:30:29Z
dc.date.available2022-03-31T16:30:29Z
dc.date.issued2022-03-30
dc.date.submitted2021-11-01
dc.identifier.issn1759-8753
dc.identifier.others13100-021-00259-7
dc.identifier.other259
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/335585
dc.description.abstractBACKGROUND: In the study of transposable elements (TEs), the generation of a high confidence set of consensus sequences that represent the diversity of TEs found in a given genome is a key step in the path to investigate these fascinating genomic elements. Many algorithms and pipelines are available to automatically identify putative TE families present in a genome. Despite the availability of these valuable resources, producing a library of high-quality full-length TE consensus sequences largely remains a process of manual curation. This know-how is often passed on from mentor-to-mentee within research groups, making it difficult for those outside the field to access this highly specialised skill. RESULTS: Our manuscript attempts to fill this gap by providing a set of detailed computer protocols, software recommendations and video tutorials for those aiming to manually curate TEs. Detailed step-by-step protocols, aimed at the complete beginner, are presented in the Supplementary Methods. CONCLUSIONS: The proposed set of programs and tools presented here will make the process of manual curation achievable and amenable to all researchers and in special to those new to the field of TEs.
dc.languageen
dc.publisherSpringer Science and Business Media LLC
dc.subjectMethodology
dc.subjectMobile DNA Tools
dc.titleA beginner's guide to manual curation of transposable elements.
dc.typeArticle
dc.date.updated2022-03-31T16:30:26Z
prism.issueIdentifier1
prism.publicationNameMob DNA
prism.volume13
dc.identifier.doi10.17863/CAM.83016
dcterms.dateAccepted2021-12-17
rioxxterms.versionofrecord10.1186/s13100-021-00259-7
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.identifier.eissn1759-8753
cam.issuedOnline2022-03-30


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