Guard-cell-targeted overexpression of Arabidopsis Hexokinase 1 can improve water use efficiency in field-grown tobacco plants.
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Authors
Głowacka, Katarzyna
Publication Date
2022-09-12Journal Title
J Exp Bot
ISSN
0022-0957
Publisher
Oxford University Press (OUP)
Type
Article
This Version
AM
Metadata
Show full item recordCitation
Acevedo-Siaca, L. G., Głowacka, K., Driever, S. M., Salesse-Smith, C. E., Lugassi, N., Granot, D., Long, S. P., & et al. (2022). Guard-cell-targeted overexpression of Arabidopsis Hexokinase 1 can improve water use efficiency in field-grown tobacco plants.. J Exp Bot https://doi.org/10.1093/jxb/erac218
Abstract
Water deficit currently acts as one of the largest limiting factors for agricultural productivity worldwide. Additionally, limitation by water scarcity is projected to continue in the future with the further onset of effects of global climate change. As a result, it is critical to develop or breed for crops that have increased water use efficiency and that are more capable of coping with water scarce conditions. However, increased intrinsic water use efficiency (iWUE) typically brings a trade-off with CO2 assimilation as all gas exchange is mediated by stomata, through which CO2 enters the leaf while water vapor exits. Previously, promising results were shown using guard-cell-targeted overexpression of hexokinase to increase iWUE without incurring a penalty in photosynthetic rates or biomass production. Here, two homozygous transgenic tobacco (Nicotiana tabacum) lines expressing Arabidopsis Hexokinase 1 (AtHXK1) constitutively (35SHXK2 and 35SHXK5) and a line that had guard-cell-targeted overexpression of AtHXK1 (GCHXK2) were evaluated relative to wild type for traits related to photosynthesis and yield. In this study, iWUE was significantly higher in GCHXK2 compared with wild type without negatively impacting CO2 assimilation, although results were dependent upon leaf age and proximity of precipitation event to gas exchange measurement.
Sponsorship
This work was supported by the project Realizing Increased Photosynthetic Efficiency (RIPE), that is funded by the Bill & Melinda Gates Foundation, Foundation for Food and Agriculture Research (FFAR), and the UK Foreign Commonwealth and Development Office under grant number OPP1172157.
Funder references
Bill & Melinda Gates Foundation (088649-17776)
Bill & Melinda Gates Foundation (via University Of Illinois) (088649-17776)
Embargo Lift Date
2023-05-20
Identifiers
External DOI: https://doi.org/10.1093/jxb/erac218
This record's URL: https://www.repository.cam.ac.uk/handle/1810/337120
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